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Ecotoxicological information

Toxicity to terrestrial plants

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Reference
Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Publicly available, well documented literature study.
Reason / purpose for cross-reference:
reference to other study
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
The germination rate of the pollen was determined by applying a modified test procedure originally developed by Kappler and Kirsten (Pollen test system. The toxicity of chemicals is determined by measuring inhibition of pollen tube growth)
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available
Analytical monitoring:
no
Details on sampling:
not applicable
Vehicle:
not specified
Details on preparation and application of test substrate:
An amount of 0.5 mg pollen and 1 mL germination medium was placed into 3.5 cm Petri dishes and held in a water saturated atmosphere.
Species:
other: Nicotiana tabacum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Plants were raised under greenhouse conditions, such that enough pollen was available throughout the year. Immediately after anthesis, the pollen was directly dusted into Eppendorf-tubes and stored at -20 °C until use. Only pollen from plants showing viability of more than 70 % was used in the experiments.
Test type:
other: germination test
Study type:
laboratory study
Substrate type:
other: petri dish + germination medium
Limit test:
no
Total exposure duration:
2 h
Test temperature:
22 °C
pH:
no data available
Moisture:
water saturated air
Details on test conditions:
Petri dishes (including pollen and germination medium) are put into small glass troughs (8.5 x 5.7 x 6.7 cm) and sealed tightly. The test substance was injected by a GC-syringe into the trough where it evaported at once. As a control the same amount of water was injected into the control troughs.
Nominal and measured concentrations:
no data available
Reference substance (positive control):
no
Species:
other: Nicotiana tabacum
Duration:
2 h
Dose descriptor:
other: ED50 (Effective Dose)
Effect conc.:
17.1 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Species:
other: Nicotiana tabacum
Duration:
2 h
Dose descriptor:
other: ED25
Effect conc.:
9.2 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Details on results:
For each concentration of the test item three seperate tests are investigated. The mean values of these three tests are used to calculate a dose response curve. The inhibition rate is calculated by setting the germination rate of the control (70 - 85 % of total amount) at 100 %.
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
ED50 value was calculated by linear interpolation.
Conclusions:
In a 2 h germination test the ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).
Executive summary:

The stimulation of pollen germination of 1,2 -dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

Description of key information

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).

Key value for chemical safety assessment

Additional information

The stimulation of pollen germination of 1,2-dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).

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