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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Publicly available, well documented literature study.
Reason / purpose for cross-reference:
reference to other study
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
The germination rate of the pollen was determined by applying a modified test procedure originally developed by Kappler and Kirsten (Pollen test system. The toxicity of chemicals is determined by measuring inhibition of pollen tube growth)
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available
Analytical monitoring:
no
Details on sampling:
not applicable
Vehicle:
not specified
Details on preparation and application of test substrate:
An amount of 0.5 mg pollen and 1 mL germination medium was placed into 3.5 cm Petri dishes and held in a water saturated atmosphere.
Species:
other: Nicotiana tabacum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Plants were raised under greenhouse conditions, such that enough pollen was available throughout the year. Immediately after anthesis, the pollen was directly dusted into Eppendorf-tubes and stored at -20 °C until use. Only pollen from plants showing viability of more than 70 % was used in the experiments.
Test type:
other: germination test
Study type:
laboratory study
Substrate type:
other: petri dish + germination medium
Limit test:
no
Total exposure duration:
2 h
Test temperature:
22 °C
pH:
no data available
Moisture:
water saturated air
Details on test conditions:
Petri dishes (including pollen and germination medium) are put into small glass troughs (8.5 x 5.7 x 6.7 cm) and sealed tightly. The test substance was injected by a GC-syringe into the trough where it evaported at once. As a control the same amount of water was injected into the control troughs.
Nominal and measured concentrations:
no data available
Reference substance (positive control):
no
Species:
other: Nicotiana tabacum
Duration:
2 h
Dose descriptor:
other: ED50 (Effective Dose)
Effect conc.:
17.1 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Species:
other: Nicotiana tabacum
Duration:
2 h
Dose descriptor:
other: ED25
Effect conc.:
9.2 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Details on results:
For each concentration of the test item three seperate tests are investigated. The mean values of these three tests are used to calculate a dose response curve. The inhibition rate is calculated by setting the germination rate of the control (70 - 85 % of total amount) at 100 %.
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
ED50 value was calculated by linear interpolation.
Conclusions:
In a 2 h germination test the ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).
Executive summary:

The stimulation of pollen germination of 1,2 -dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

Description of key information

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).

Key value for chemical safety assessment

Additional information

The stimulation of pollen germination of 1,2-dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).