1,2-dichloroethane

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Publicly available, well documented literature study.

Reason / purpose for cross-reference:

reference to other study

Qualifier:

no guideline followed

Deviations:

not applicable

Principles of method if other than guideline:

The germination rate of the pollen was determined by applying a modified test procedure originally developed by Kappler and Kirsten (Pollen test system. The toxicity of chemicals is determined by measuring inhibition of pollen tube growth)

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data available

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

not specified

Details on preparation and application of test substrate:

An amount of 0.5 mg pollen and 1 mL germination medium was placed into 3.5 cm Petri dishes and held in a water saturated atmosphere.

Species:

other: Nicotiana tabacum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

Plants were raised under greenhouse conditions, such that enough pollen was available throughout the year. Immediately after anthesis, the pollen was directly dusted into Eppendorf-tubes and stored at -20 °C until use. Only pollen from plants showing viability of more than 70 % was used in the experiments.

Test type:

other: germination test

Study type:

laboratory study

Substrate type:

other: petri dish + germination medium

Limit test:

no

Total exposure duration:

2 h

Test temperature:

22 °C

pH:

no data available

Moisture:

water saturated air

Details on test conditions:

Petri dishes (including pollen and germination medium) are put into small glass troughs (8.5 x 5.7 x 6.7 cm) and sealed tightly. The test substance was injected by a GC-syringe into the trough where it evaported at once. As a control the same amount of water was injected into the control troughs.

Nominal and measured concentrations:

no data available

Reference substance (positive control):

no

Species:

other: Nicotiana tabacum

Duration:

2 h

Dose descriptor:

other: ED50 (Effective Dose)

Effect conc.:

17.1 other: mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Species:

other: Nicotiana tabacum

Duration:

2 h

Dose descriptor:

other: ED25

Effect conc.:

9.2 other: mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Details on results:

For each concentration of the test item three seperate tests are investigated. The mean values of these three tests are used to calculate a dose response curve. The inhibition rate is calculated by setting the germination rate of the control (70 - 85 % of total amount) at 100 %.

Results with reference substance (positive control):

no data available

Reported statistics and error estimates:

ED50 value was calculated by linear interpolation.

Conclusions:

In a 2 h germination test the ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).

Executive summary:

The stimulation of pollen germination of 1,2 -dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

Description of key information

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).

Additional information

The stimulation of pollen germination of 1,2-dichloroethane to Nicotiana tabacum pollen, was determined in a 2 -hour germination test. Treatments consisted of a dilution water control and at test substance concentrations.

The ED50 of Nicotiana tabacum pollen was determined to be 17.1 mg 1,2-dichloroethane/cm2 (no 95 % Confidence Intervals are available).