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EC number: 203-458-1
CAS number: 107-06-2
1,2 dichloroethane was examined for its
possible cytogenetic effects (induction of chromosomal damage) using the
micronucleus test, similarily conducted according to OECD Test Guideline
474 (Mammalian Erythrocytes Micronucleus Test) . Micronucleus (MN)
induction in peripheral blood was examined in 10 male and female mice
(lymphoma prone Eµ - PIM 1 transgenic mice) per dose after a repeated
dose of 1,2 -dichloroethane in corn oil administered by gavage (5 mL/kg
bw) once a day in concentrations of 100 and 200 mg/kg bw (in males) and
150 and 300 mg/kg bw (in females) up to week 6. After week 6 until the
end of the exposure period (week 41) the top doses were reduced,
therefore male animals were only administered with 100 mg/kg bw and
females were only administered with 150 mg/kg bw (due to mortality and
treatment related weight gain). Two negative controls were performed, a
vehicle control and a “true” negative control with methyl cellulose.
Peripheral blood was collected at week 14 and at terminal sacrifice (41
week) and analysed a week later (week 42). Stained slides (acridine
orange staining) from 10 animals per treatment group were analysed for
the number of micronucleated cells per 1000 NCE (normochromatic
erythrocytes) and the number of PCE (polychromatic erythrocytes) per
1000 erythrocytes (Since exposure was discontinued one week before the
terminal harvest of mice, only NCE were scored for micronuclei because
of the short residence time of polychromatic erythrocytes.
Results showed no micronucleus induction (in
normochromatic erythrocytes) after 14 weeks or 41 weeks of 1,2
-dichloroethane exposure and no polychromatic erythrocyte suppression
was detected in the blood after 14 weeks or 41 weeks of exposure to the
test substance. It can therefore be concluded that under the study
conditions, 1,2 -dichloroethane induced no cytogenetic damage/chromosome
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