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EC number: 203-458-1
CAS number: 107-06-2
Single micro-organismIn a cell multiplication inhibition test the toxicity threshold for the model organism Pseudomonas putida for 1,2-dichloroethane was determined to be 135 mg/L.Activated sludgeBased on the results of the key study the IC50 of 1,2-dichloroethane on activated sludge is 2.78 g/L.
In a cell multiplication inhibition
test (Bringman & Kuehn, 1976) the toxicity threshold for the model
organism Pseudomonas putida for 1,2-dichloroethane was determined
to be 135 mg/L. This study was designed to determine the effect of
1,2-dichloroethane on micro-organisms in aequous solution by measuring
the inhibition of cell growth after exposure of 16 h. The study was
taken as key study, as it was the best documented study, therefore it
was considered as most reliable study.
The key study for a multi-species
inhibition test ( Tang, Blum & Speece, 1990) was designed to determine
the effects of 1,2-dichloroethane on sewage sludge micro-organisms by
measuring the respiration rate after exposure to the test substance
after a 3 h contact time. Two protocols were assayed: the regular OECD
209 and a serum bottle, aimed to prevent losses of volatiles. Based on
the results of this study the IC50 of 1,2-dichloroethane on activated
sludge is 35.5 g/L in the regular test and 2.78 g/L in the serum bottle
design. The study is selected as the key study for microbial
According to the Guidance (R.7.8.18),
"In case of multiple microbial inhibition data, the PNECstp is
usually derived from results obtained for the most sensitive test system
available, regardless of whether this is a test with activated sludge,
relevant single bacterial spicies or ciliated protozoa". Consequently,
the study on Pseudomonas putida NOEC = 135 mg/L should normally be used
to determine the PNECstp. However, table R.10 -6 in Guidance recommends to
use Bringmann & Kuehn data only if no other test is available (it is known
that volatile substances were not properly addressed by their test procedure).
Finally, the IC50 = 2.78 g/L will be used for PNECstp derivation.
Following additional information on
toxicity to microorganism is available:
158 mg/L (5 min)
Qureshi, A.A. (1982)
1000 mg/L (15 min)
Hermens, J. et al. (1985)
5300 mg/L TT (30 min)
Niemitz, W. & Trenel, J. (1979)
583 mg/L EC10 (30 min)
Trenel, J. & Kuehn, R. (1982)
1127 mg/L TT
Bringmann, G. & Kuehn, R. (1980)
laboratory sewage sludge
50 mg/L (4 d)
Bringmann, G. & Kuehn, R. (1980): Water
Research 14, 231 - 241.
Hermens, J. et al. (1985): Ecotoxicol.
Environ. Safety 9, 17-25.
Niemitz, W. & Trenel, J. (1979): Inst.Wasser-,
Boden-, Lufthyg. Bundesgesundheitsamt, Berlin, 1 - 10.
Qureshi, A.A. et al. (1982): ASTM
Spec. Techn. Publ. 766,|179 - 195.
Stuckey,et al.(1980): J. Water Pollut.
Control Fed. 52, 720 - 729.
Trenel, J. & Kuehn, R. (1982): Inst.
Wasser-, Boden-, Lufthyg. Bundesgesundheitsamt, Juli 1982, 12 - 14, 25 -
27, Anlage 2 (23 - 28), Anlage 4 (1 - 5).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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