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EC number: 200-772-0
CAS number: 72-17-3
RS-Freetext:When the culture medium was first acidified by the lacticacid dose and neutralized to pH 6.4 or when medium iscontaining 30 mM HEPES as buffer, lactic acid wasnon-clastogenic.Pseudo-positive reactions are seen as a result ofnon-physiological low pH.
Using Chinese hamster ovary Kl cells, chromosomal aberration tests were
carried out with formic acid, acetic acid and lactic acid, and the
relationship between the pH of the medium and the clastogenic activity
was examined. The medium used was Ham's F12 supplemented with 17 mM
NaHC03 and 10% fetal calf serum. All of these acids induced chromosomal
aberrations at the initial pH of ca. 6.0 or below (about 10-14 mM of
each acid) both with and without S9 mix. Exposure of cells to about pH
5.7 or below (about 12-16 mM of each acid) was found to be toxic. When
the culture medium was first acidified with each of these acids and then
neutralized to pH 6.4 or pH 7.2 with NaOH, no clastogenio activity was
observed. Using F12 medium supplemented with 34 mM NaHC03 as a buffer,
no clastogenic activity was observed at doses up to 25 mM of these acids
(initial pH 5.8-6.0). However, it was found that about 10% of the cells
had aberrations at pH 5.7 or below (27.5-32.5 mM of each acid).
Furthermore, when 30 mM HEPES was used as a buffer, chromosomal
aberrations were not induced at doses up to 20 mM formic acid and acetic
acid (initial pH 7.0-7.1), and at doses up to 30 mM lactic acid (initial
pH 6.6). In the initial pH range of 6.4-6.7 (25-32.5 mM of each acid),
chromosomal aberrations were observed. The above results show that these
acids themselves are non-clastogenic, and the pseUdo-positive reactions
attributable to non-physiological pH could be eliminated by either
neutralization of the treatment medium or enhancement of the buffering
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