Biphenyl-2-ol

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.13 (Bioconcentration: Flow-through fish test)

Version / remarks:

Cited as Directive 98/73/EC, C.13

GLP compliance:

yes

Radiolabelling:

no

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the stock solution, the coarsly-particled test substance was pulverized, then weighed into water, treated in an ultrasonic bath for 1 hour and finally stirred with a magnetic stirrer for another hour. Stock solution was prepared daily.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Bio International B.V., Netherlands
- Age at study initiation: 4 month
- Length at study initiation: 2.5-3.5 cm
- Feeding during test
- Food type: Tetra Min fish food, ground
- Amount: 1% of body weight, applicated in 2 portions
- Frequency: daily

KEEPING CONDITIONS
- Health during Keeping: < 5%
- Medical treatment: none

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

53 h

Total depuration duration:

19 h

Test temperature:

20.0 - 20.4 °C

pH:

7.8 - 7.9

Dissolved oxygen:

8.7 - 9.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: fish tank, 41 x 25 x 30 cm, 25 L
- Renewal rate of test solution (frequency/flow rate): about 20 times per day
- No. of organisms per vessel: 50
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: syntehtic fresh water in accordance with ISO 7346
- Intervals of water quality measurement: temperature, oxygen contnent and pH during acclimation, uptake and depuration phase

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/ 8 hours dark

ANALYSIS
- Analysis: Analysis of test and control media as well as fish samples were conducted 2, 6, 23, 30 and 48 h after test start. After beginning of the depuration phase (53 h) analysis was carried out in water and in fish samples after 54 and 72 h.
- Detection limit test medium: 0.67 µg/L
- Detection limit test fish: 16.1 µg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 5, 50 µg/L

Lipid content:

14.7 other: % ww

Time point:

start of exposure

Remarks on result:

other: control fish

Lipid content:

21.1 other: % ww

Time point:

other: end of depuration phase

Remarks on result:

other: control fish

Lipid content:

23.4 other: % ww

Time point:

other: end of depuration phase

Remarks on result:

other: 5 µg/L treatment group

Lipid content:

22.9 other: % ww

Time point:

other: end of depuration phase

Remarks on result:

other: 50 µg/L treatment group

Type:

BCF

Value:

21.7

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:both

Type:

BCF

Value:

114

Basis:

other: lipid content of ww

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:5 µg/L

Type:

BCF

Value:

115

Basis:

other: lipid content of ww

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:50 µg/L

Details on results:

- Mortality of test organisms: none
- Behavioural abnormalities: none
- Mortality and/or behavioural abnormalities of control: none

Stady state:

The time to reach steady-state conditions during the exposure period was very short. The first samples (water, fish) taken only two hours after the onset of the uptake phase yielded a CF/CW ratio (= steady-state BCF) of 20.8 in the lower concentration and 17.6 at the higher test concentration, respectively. The ratios for the low test concentration were 17.6 (after 6 h), 28.8 (after 23 h), 21.0 (after 30 h) and 20.5 (after 48 h). At the higher test concentration the respective CF/CW ratios were 25.0 (after 6 h), 22.0 (after 23 h), 22.3 (after 30 h) and 21.5 (after 48 h).

Depuration phase:

One hour after turning to clean water conditions the test substance concentration at the lower test level (5 µg a.i./L) had fallen below the analytical detection limit, both in water and in fish samples. At the higher test level (50 µg a.i./L), analytical values fell below the detection limits (water fish) 19 h after the start of the depuration phase, thus, not providing an appropriate data basis for the determination of the depuration rate constant (k2) nor of the uptake rate constant (k1).

Description of key information

The BCF value of 21.7 indicates a negligible potential of OPP to bioaccumulate.

Additional information

To evaluate the bioaccumulation potential of OPP (CAS-No. 90-43-7) a flow-through study according to Directive 67/548/EC, C.13 was conducted. The Test was carried out with individuals of zebra fish (Danio rerio). A dosing system was used to maintain water concentrations (nominal) of 5.0 and 50 µg OPP/L for the 53-h exposure period. After exposure, the test fish were placed in clean water to determine the depuration of the test substance (Caspers, 1999).

At both test substance concentrations, the arithmetic means of five consecutive steady-state BCF were 21.7, indicating a negligible potential of the test substance to bioaccumulate. The achievement of steady-state conditions during the uptake (53 h exposure) phase as well as the subsequent depuration (19 h) were rapid processes.

The steady-state bioconcentration factor (BCF) of 21.7 indicates a negligible potential of OPP to bioaccumulate.