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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms
Adequacy of study:
other information
Total exposure duration:
6 h
Duration:
6 h
Dose descriptor:
NOEC
Effect conc.:
1.7 mg/L
Nominal / measured:
nominal
Executive summary:

A oxygen-consumption test with acetone or nonylphenol as solubilizer (Hüls 1988) showed no effects on microorganisms up to a concentration level of 1.7 mg DIBP/L.

Endpoint:
toxicity to microorganisms
Adequacy of study:
other information
Test organisms (species):
Tetrahymena pyriformis
Dose descriptor:
EC100
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Basis for effect:
growth inhibition
Executive summary:

The study (Yoshizawa et al. 1977) showed a complete growth inhibition at a DIBP level of 50 mg/L.

Endpoint:
toxicity to microorganisms
Type of information:
other: Well documented report, based on scientific literature.
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline is reported.
Qualifier:
no guideline available
Principles of method if other than guideline:
No guideline is reported.
GLP compliance:
not specified
Remarks on result:
other: see table below

Toxicity of DBP to microorganisms‌‌


No.

Species

Result (mg/l)

Method

Reference

1

Pseudomonas putida

>10 (30 min NOEC)

DIN 38412/27

BASF (1989)

2

Pseudomonas putida

>10 (6 h EC10)

Other

Hüls (1995)

3

Tetrahymena pyriformis

2.2 (24 h EC50)

Other

Yoshioka et al. (1985)

4

Photobacterium phosphoreum

10.9 (30 min EC50)

Microtox

Tarkpea et al. (1986)

In both Pseudomonas putida tests no effects of DBP were found even at concentrations above the water solubility of the substance. The low toxicity of DBP to bacteria is supported by the results of the biodegradability test (Huls study, 1995; modified Sturm test). In this test, showing ready biodegradability of DBP, a test concentration of 21.7 mg/l was used.

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Test organisms (species):
Tetrahymena pyriformis
Test type:
static
Total exposure duration:
24 h
Test temperature:
30 °C
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2.2 mg/L
Details on results:
cf. Table 1, line 9
Conclusions:
An EC50 of 2.2. mg/l was measured.
Executive summary:

A test was developed using Tetrahymena pyriformis in order to determine the toxicity of various chemicals. Pre-cultured T. pyriformis was exposed for 24 h at 30°C to various concentrations of chemicals, and the number of T. pyriformis surviving were then counted. The concentration of the chemical, at which the proliferation of T. pyriformis was restricted to one-half of the blank test (EC50), was determined.

Description of key information

In both Pseudomonas putida tests no effects of DBP were found even at concentrations above the water solubility of the substance. The low toxicity of DBP to bacteria is supported by the results of the biodegradability test.
For the EC50 value the most sensitive endpoint (Tetrahymena pyriformis) has been chosen:

Key value for chemical safety assessment

EC50 for microorganisms:
2.2 mg/L
EC10 or NOEC for microorganisms:
10 mg/L

Additional information