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Ecotoxicological information

Biotransformation and kinetics

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Administrative data

Endpoint:
biotransformation and kinetics
Adequacy of study:
other information

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983

Materials and methods

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Transformation products:
yes
Identity of transformation products
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
100-54-3
Identifier:
CAS number
Identity:
100-54-3

Any other information on results incl. tables

Di-n-butylphthalate was rapidly converted by rainbow traut liver microsomes to unextractable compound present in the aqueous phase (Table 16). Approximately 40% of the radiolabeled compound was converted within 15 min, and 60% within 1 hr. Irreversible protein binding occurred throughout the 2 hr incubation with 14C labeled di-n-butylphthalate. Approximately 9% of the radiolabeled compound was bound to the trout liver microsomes during a 2 hr incubation with 7% of the total bound in the first 15 min of incubation.

Radiolabeled di-n-butylphthalate was converted more slowly by Daphnia PMS during a 1 hr incubation than by trout liver microsomes. Only about 10% of the radiolabeled compound was converted (Table 16) to water soluble hexane unextractable compounds. Less than 1% of the di-n-butylphthalate was irreversibly bound to protein.

Applicant's summary and conclusion

Conclusions:
The study showed that protein binding of di-n-butylphthalate occurred in rainbow traut liver microsomes and to a more limited extent in Daphnia PMS.
Executive summary:

Radiolabeled (14C) di-n-butylphthalate was incubated with a protein substrate and NADPH generating system to determine metabolism and irreversible protein binding of di-n-butylphthalate in rainbow traut liver microsomes and daphnia PMS.