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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Adequacy of study:
other information
Test organisms (species):
Pimephales promelas
Test type:
flow-through
Total exposure duration:
96 h
Test temperature:
25 (+/- 1) degrees Celcius
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.9 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
0.73 mg/L
Basis for effect:
behaviour
Remarks:
= imbalance
Executive summary:

The study (Geiger at al 1985) measured EC50 and LC50 effect levels of DIBP for fathead minnows (pimephales promelas).

Endpoint:
short-term toxicity to fish
Adequacy of study:
other information
Qualifier:
according to
Guideline:
other: DIN 38412, part 15
Test organisms (species):
Leuciscus idus
Test type:
static
Total exposure duration:
48 h
Test temperature:
20 (+/- 1) degrees Celcius
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
2.5 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
4.2 mg/L
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
6 mg/L
Executive summary:

The study (Hüls 1989) measured LC0, LC50 and LC100 effect levels of DIBP for orfes (leuciscus idus).

Endpoint:
short-term toxicity to fish
Adequacy of study:
other information
Test organisms (species):
Lepomis macrochirus
Details on test organisms:
Test animals utilized were young of the year bluegill (L. macrochirus) obtained from commercial fish suppliers within the continental United States. Wet weight of these fish ranged from 0.32-1.2 g.
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
32-48 mg/l CaCO3
Test temperature:
21-23 C
pH:
6.7-7.8
Dissolved oxygen:
7.0-8.8 mg/l
Details on test conditions:
Upon receipt at Bionomics, each test population was held in a separate tank receiving well-water at a minimum flow rate of 4 volume replacements per day. Chemical and physical characteristics of this well-water were measured weekly, revealing a total hardness of 28-44 mg/L CaC03, a total alkalinity of 20-30 mg/L CaCO3, a pH of 6.7-7.4, a dissolved oxygen concentration of 5.3-7.0 mg/L, a specific conductance of 95-170 ~mbos/cm and a temperature of 20-24C. All fish were fed ad libitum daily with dry, pelleted food and once weekly with raw ground beef liver. All feeding was discontinued for 48 h prior to testing. No population was used in toxicity tests if the mortality exceeded 3% during this 48-h period.
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
2.1 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 - 1.4 mg/L
Conclusions:
A 24h LC50 for DBP was established at 2.1 mg/l and a 96h LC50 at 1.0-1-4 mg/l.
Executive summary:

The study tested several chemicals on bluegills. Only the effects of DBP are documented here.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Conclusions:
Measured effect concentrations LC50 (96h, 95% C.I.) range between 0.35 mg/l (Perca flavescens) and 7.3 mg/l (Leuciscus idus).
Executive summary:

The DBP short-term toxicity studies with fish are summarised as following:

No.

Species

96h LC50(mg/l) 95%C.I.

Method

Reference

1

Brachydaniorerio

2.2(1.3-2.5)

semistatic(EEC92/69C1)

Hüls(1994a)

2

Pimephalespromelas

0.9(0.7-1.2)

flowthrough(EG&GBionomics,1981)

CMA(1984)

3

Pimephalespromelas

2.0(1.3-2.9)

flowthrough(EPA-60018-81-011)

McCarthyandWhitmore(1985)

4

Pimephalespromelas

1.3

static(APHA,1971)

MayerandSanders(1973)

5

Pimephalespromelas

3.0(2.6-3.4)

static(EG&GBionomics,1982)

CMA(1984)

6

Pimephalespromelas

1.1(1.0-1.2)

static

Geigeretal.(1985)

7

Oncorhynchusmykiss

1.6(1.1-2.2)

flowthrough(EG&GBionomics,1981)

CMA(1984)

8

Oncorhynchusmykiss

6.5

static(APHA,1971)

Hudsonetal.(1981)

9

Ictaluruspunctatus

0.46

flowthrough

MayerandEllersieck(1986)

10

Ictaluruspunctatus

2.9

static(APHA,1971)

MayerandSanders(1973)

11

Lepomismacrochirus

0.9(0.7-1.0)

static(EG&GBionomics,1982)

CMA(1984)

12

Lepomismacrochirus

0.7

static(APHA,1971)

MayerandSanders(1973)

13

Lepomismacrochirus

1.2(1.0-1.4)

static(EPA,1975)

CMA(1984)

14

Lepomismacrochirus

1.2(1.0-1.4)

static

Buccafuscoetal.(1981)

15

Percaflavescens

0.35

flowthrough

MayerandEllersieck(1986)

16

Leuciscusidus

7.3(4.6-10)

static(DIN38412,1982)

CMA(1984)

Endpoint:
short-term toxicity to fish
Adequacy of study:
other information
Test organisms (species):
other: rainbow trout, fathead minnow, channel catfish, bluegill
Total exposure duration:
96 h
Test temperature:
rainbow trout: 12 C
fathead minnow, channel catfish, bluegill: 17 C
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.6 mg/L
Remarks on result:
other: 95% CI: 1.2-5.3; rainbow trout
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.3 mg/L
Remarks on result:
other: 95% CI: 0.3-5.5; fathead minnow
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.9 mg/L
Remarks on result:
other: 95% CI: 1.4-6.1; channel catfish
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.7 mg/L
Remarks on result:
other: 95% CI: 0.3-1.3; bluegill
Executive summary:

The summarized LC50 levels for four fish species, as documented in the handbook, are listed here.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Reason / purpose:
reference to same study
Test organisms (species):
Oryzias latipes
Details on test organisms:
Orizias latipes (ca. 3 cm, 0.3 g) was obtained from the market and acclimated for at least 1 week in dechlorinated water at 20°C (total hardness was about 80 mg/liter).
Total exposure duration:
48 h
Hardness:
80 mg/l
Test temperature:
20 +/-1 °C
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.63 mg/L
Nominal / measured:
meas. (not specified)

The results obtained are summarized in Table 2.

Changes in the oxygen uptake rate of 0. latipes in 4 hr are illustrated in Fig. 2.

Conclusions:
An LC50 of 0.63 mg/l was measured after 48h.
Executive summary:

Biological tests using Orizias latipes (LC50 and oxygen uptake test) were carried out in order to clarify the mutual relationship of test methods.

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