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EC number: 201-557-4
CAS number: 84-74-2
The effect of DBP on photosynthesis in protoplasts prepared from
Protoplasts were used because we wanted to compare photosynthesis in
higher plant with that of an alga, without interference by the long
range transport of metabolites typical for higher plants. Photosynthesis
was measured either as 14CO2-reduction or as CO2-dependent oxygen
evolution. The chlorophyll concentrations were different in the two
types of experiments (Tab. 1). DBP inhibited photosynthesis at
concentrations higher than lO^-5 M (Fig. 3).
The effect of DBP on electron transport in isolated thylakoids from
As photosynthesis was inhibited by DBP in both algae and protoplasts, we
investigated in what photosynthetic system(s) the effect was localized.
By choosing isolated thylakoids as a test system and by comparing the
results obtained on different organisational levels (cells, protoplasts,
thylakoids) we should be able to assign the effect of DBP to a certain
The effects of DBP on coupled and NH4CI-uncoupled basal electron
transport in photosystem II + I are shown in Fig. 4. Electron transport
was inhibited at DBP concentrations higher than 10^-4 M, the uncoupled
system being the most sensitive system with an IC50 value of 3 X 10^-4.
With a photosystem I assay (methylviologen as electron acceptor and
dichlorophenolindophenol/ascorbate as electron donor couple) no effect
was observed with DBP concentrations as high as 10^-3 (results not
shown). Reactions in
photosystem II are thus being affected by DBP. In order to determine a
chlorophyll independent IC50 value the method of Tischer and Strotmann
(1977) was used. Here the ferricyanide dependent oxygen evolution in
thylakoids devoid of coupling factor is measured at different
chlorophyll concentration. By extrapolating to zero chlorophyll
concentration the IC50 value in DBP treated thylakoids was found to be
2.5 x 10^-5 M.
This is part of a study conducted on algae, barley and spinat. Only the
effects on barley and spinach are documented here. Those on algae can be
found under section 6.1.5.
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