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EC number: 201-549-0
CAS number: 84-65-1
aim of the study was to investigate acute toxic effects of the test
substance, Anthraquinone, after a single oral administration to Wistar
testing was performed according to the Method B.1 tris: Acute Oral
Toxicity - Acute Toxic Class Method, Council Regulation (EC)
No.440/2008, published in O.J. L 142, 2008.
test substance was administered in a single dose as solution in vehicle
(olive oil), given orally via gavage to two groups of three female
dosing was performed sequentially in two groups of three females: group
No. 1 - first step using the starting dose of 2000 mg/kg of body weight
and group No.2 - second step using the same dose.
test substance administered at the dose of 2000 mg/kg caused no death of
animals. No clinical signs of intoxication were observed in all animals.
pathologic macroscopic changes were diagnosed during pathological
examination in all animals
to the study results the value of LD50 of the test substance for female
rats is higher than 2000 mg/kg of body weight.
classification of the test substance toxicity was performed according to
the Directive 67/548/EEC, Annex VI. part 3.1.5. and 3.2.
on the test results and according to the EC criteria for classification
and labelling requirements for dangerous substances and preparations the
test substance Anthraquinone did not fall into any of quoted categories
of toxicity and has no obligatory labelling requirement in this respect.
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