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EC number: 201-549-0
CAS number: 84-65-1
studies are waived because of high insolubility of anthraquinone in
closed bottle test according to EU method C.4E, comparable to the
OECD TG 301D with inoculum containing mixed population of microorganisms
obtained from secondary effluent of wastewater treatment plant the
biodegradation of Anthraquinone was investigated and inhibition control
(toxicity test) was done.
test was performed at temperature of 20±1°C with the pH values of
solution 7-8 at the beginning of the test. In this 28-days study the
degradation of 62.0% of the Anthraquinone was attained in the end of
A., M.Sc., 2009).
the toxicity test, after 14 days of incubation the degradation of test
and reference substance was 47.2%, therefore the AQ is
not inhibiting for microorganisms in used inoculum (Nachtigallová
A., M.Sc., 2009).
the AQ has a limited solubility in water a direct dosing of dispersion
into each bottle was implemented. The dispersion of the test substance
was prepared as 0.1g to 1000 ml mineral medium. 4.7 ml of dispersion
were dosed into individual bottles (volume approximately 283.4 mL). The
tested concentration at which toxicity to the inoculum was ruled out
(NOEC) was 1.66 mg/L (nominal test concentration).
of study is above
water solubility, thus the
test result has to be considered as unreliable.
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