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EC number: 201-549-0
CAS number: 84-65-1
Preliminary test showed that it could be reasonable to expect that estimated EC50 will be higher than solubility of the test substance. Water solubility of the test substance is 0.0746 mg.L-1, and the average determined concentration in M4 medium was 0.0539 mg.L-1. 21 d–EC50 > 0.0539 mg.L-1 (average measured concentration)When the assumed value of EC50 is higher than solubility of the test substance in M4 medium, no further testing is needed and the result obtained in preliminary test presents the result of whole study.
substance, Anthraquinone, was tested in reproduction test on Daphnia
magna according to submitted testing proposal.
was performed according to method C.20 – Daphnia magna Reproduction
Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
test substance is poorly soluble in M4 medium at concentrations needed
for the test, the saturated solution of the test substance in M4 medium
was prepared for preliminary test. The saturated solution was prepared
by adding 1 mg.L-1of the test substance to M4 medium. The saturated
solution was stirred for period 72 hours on a shaft stirrer and
consequently filtered through 0.45 μm filter.
preliminary test was performed in range of test substance concentration
1000 – 125 mL.L-1. The test was performed as semi-static, frequency of
the test solutions renewal was three times a week. Samples for
analytical determination were taken at each test concentration several
times during of the test (before and after renewal of the test
highest calculated reduction in reproductive output was 1.8 %, the exact
values of EC50 could not be calculated on the basis of data acquired.
Test result is given in the form of a range.
evaluations and results the measured concentration of the test substance
results: 21 d–EC50>0.0539
mg.L-1 (average measured concentration)
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