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EC number: 201-549-0
CAS number: 84-65-1
In closed bottle test, comparable to
the OECD TG 301D with inoculum containing mixed population of
microorganisms obtained from secondary effluent of wastewater treatment
plant the biodegradation of Anthraquinone
(purity of main component 9,10 -Anthraquinonewas
98.9%) was investigated.
The start of degradation, which
point is taken as the time when 10% of the substance has been degraded,
was by 7thday of the test. Degradation of 51.9% was achieved
ten days after the start of degradation.
In this 28-days study the
degradation of 62.0% of Anthraquinone was attained in the end of study (Nachtigallová
A., M.Sc., 2009).
According to Regulation No
1272/2008 Anthraquinone is considered not ready biodegradable, because
according to Article 126.96.36.199.5. of this Regulation the substance is
considered rapidly degradable in the environment if in 28-day ready
biodegradation studies, at least the following level of degradation is
achieved for test based on oxygen depletion or carbon dioxide
generation: 60 % of theoretical maximum. These levels of biodegradation
must be achieved within 10 days of the start of degradation which point
is taken as the time when 10 % of the substance has been degraded.
result in a test for ready biodegradability does not mean that the
chemical will not be degraded under relevant environmental conditions
and persist in the environment. A failed ready biodegradability test
indicates that further testing under less stringent test conditions
should be considered at the next level.
In terms of risk assessment
Anthraquinone is considered to be readily biodegradable but failing 10-d
For PBT assessment according to
Guidance on information requirements and chemical safety assessment
Chapter R.11: PBT Assessment, 2008: “if the substance is readily
biodegradable, or if the criteria for ready biodegradability are
fulfilled with exception of the 10-day window, there is no reason to
perform further biodegradation tests for the PBT/vPvB assessment. The
conclusion is that the substance is not fulfilling the criteria for
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