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EC number: 201-549-0
CAS number: 84-65-1
results of experiments are summarized in tables IN Final report. The
tables contain the dose applied per plate inµg
(doses were applied to plates in volume 0.1 mL), amount of S9 per plate
in µL numbers of revertants in single plates, average number of
revertants per plate x and its standard deviation sd and ratio of
revertants at tested dose (Rt) to number of revertants
at negative control (Rc, dimethylsulfoxide).
substance Anthraquinone was assayed for the mutagenicity by the
Bacterial Reverse Mutation Test. The test was performed according to EU
method B.13/14 Mutagenicity – Reverse mutation test using bacteria ,
which is analogous to the OECD Test Guideline No. 471.
Four indicator Salmonella typhimurium strains TA
TA 1537 and one indicator Escherichia coli WP2 uvrA strain were used.
The test substance was diluted in dimethylsulfoxide and assayed in doses
which were applied to plates in volume of 0.1 mL.
series of experiments were performed with each strain - without
metabolic activation and with a supernatant of rat liver and a mixture
the arrangement given above, the test substance Anthraquinone was
nonmutagenic for all the used bacterial strains with as well as without
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