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Experimental short term toxicity studies are waived because of highly insolubility of anthraquinone in water (In accordance with REACH Regulation (EC) No 1907/2006, Annex VII and Annex VIII, the studies do not need to be conducted if the substance is highly insoluble in water. Seeing that the solubility is 74.6 micrograms per liter, substance meets the criteria for insolubility). The long-term aquatic toxicity study was performed according to testing proposal (study was performed according to method C.20 – Daphnia magna Reproduction Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008).

There are five acute toxicity studies available (three for fish, one acute toxicity study to freshwater invertebrates and one for marine organisms) with reliable results and where selection of the test concentrations was based on the limit of water solubility of anthraquinone. Based on these data, it was established that under the conditions maintained during the studies anthraquinone is not acutely toxic to fish, daphnids and eastern oysters at and below the material's limit of water solubility.

The same result was obtained in long-term reproduction test on Daphnia magna, where the anthraquinone do not display toxicity at and below the water solubility limit. The highest calculated reduction in reproductive output was 1.8 % and the exact values of EC50 could not be calculated on the basis of data acquired (21 d–EC50>0.0539 mg.L-1 (average measured concentration)). The assumed value of EC50 is higher than solubility of the test substance in testing medium, no further testing is needed.

No reliable standard microbial inhibition test data are available. Results of toxicity study of anthraquinone to microorganisms are above water solubility, thus the test result has to be considered as unreliable.

The considered substance is found to be nontoxic in acute and chronic tests at its limit of water solubility. Estimation of PNECs value is therefore impossible, and also the estimated L(E)C50 can only be used as an indication of the aquatic toxicity of the chemical considered.