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EC number: 201-549-0
CAS number: 84-65-1
test substance, Anthraquinone, was tested for the assessment of eye
irritation/corrosion effects using albino rabbit (New Zealand Albino
test was performed according to the Method B.5 Acute Toxicity: Eye
Irritation/Corrosion. Council Regulation (EC) No.440/2008, published in
O.J. L 142, 2008.
test was performed initially using one animal. Because a corrosive
effect was not observed in initial test, the response was confirmed
using two additional animals.
changes described further were observed on eye of animals. The effects
were detected at all three animals at 1 hour after application, in two
animal the lesions persisted to 24 h after application.
at 1 hour after application: redness of conjunctivae – diffuse, crimson
colour, individual vessels not easily discernible; chemosis – some
swelling above normal.
at 24 hours after application: conjunctivae – some blood vessels
at 48 hours after application: no alterations
clinical signs of systemic intoxication were detected.
of eye irritation after single application demonstrated, that the test
substance, Anthraquinone, is not irritating for eye of rabbit.
classification of the test substance eye irritation/corrosion effect was
performed according to Directive 67/548/EEC, Annex VI (part 126.96.36.199.)
on the test results and according to the EC criteria for classification
and labelling of dangerous substances and preparations the test
substance does not have to be classified for eye irritation/corrosion
and has no obligatory labelling requirement for eye irritation/corrosion.
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