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EC number: 215-252-9
CAS number: 1315-01-1
purpose of the micronucleus test is to identify substances that cause
cytogenetic damage which results in the formation of micronuclei
containing lagging chromosome fragments or whole chromosomes.
For this study 50 male and 50 female rats (8-12 weeks old) were used,
divided into 5 groups: dose group D1 (500 mg/kg), D2 (1000 mg/kg), D3
(2000 mg/kg), group C (negative control) and group CP (positive
control-cyclophosphamide), each including 20 animals t 10 males and 10
females). Samples of bone marrow were taken at 24 and 48 hours after the
Each animal in treated groups received the appropriate dose of Test item
suspension orally by gavage in the volume of 1 mL per 100 g body weight.
The animals in the negative control group received the appropriate
volume of saline solution by the same way. The animals in the positive
control group were administered cyclophosphamide solution at the dose of
20 mg/kg by a single intraperitoneal application in the volume of 1 mL
per 100 g body weight.
All the rats were observed for clinical signs, morbidity or mortality
during acclimation, before administration and after administration in
time points 0 (immediately after dosing), 0.5, 1, 2, 4 and 24 hours
post-dose and then before necropsy.
All the rats were individually weighed at delivery and prior to the
administration in order to calculate dose levels.
No changes in health status and condition of the animals in any of the
groups were recorded during the acclimation and during the study.
No significant changes of mean body weight were observed in the animals
during the study.
All the values of number of normochromatic erythrocytes with micronuclei
in all dose groups (D1, D2 and D3) were within the reference range for
negative control group. No statistically significant higher values of
number of normochromatic erythrocytes with micronuclei in any of the
dose groups (up to 11 of micronuclei) as compared to negative control
group (up to 13 micronuclei) were noted. Statistically significant
differences were observed in the positive control group (normochromatic
erythrocytes with micronuclei -up to 38 of micronuclei) as compared to
increase was found in the frequency of micronucleated polychromatic
erythrocytes in treated animals as indication of induced chromosome
damage. Tin sulfide does not induce damage to the chromosomes or the
mitotic apparatus of erythroblasts.
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