Registration Dossier

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
A comparative study of the toxicity and inhibitory effects of inorganic tin compounds on various biological subjects
Author:
Farasova, Agata
Year:
1994
Bibliographic source:
Biologia (Bratislava) 49(3): 307-311

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EPA OPPTS 850.4200 (Seed Germination/Root Elongation Toxicity Test)
Deviations:
not specified
Remarks:
no replication, but higher number of seeds exposed so that total number of seeds is above teh recommendation of the guideline
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Details on test material:
SnCl2*2H2O p.a. from Lachema, Brno, Czech Republic

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Species:
Sinapis alba
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
seed germination/root elongation toxicity test
Study type:
laboratory study
Substrate type:
filter paper
Limit test:
no
Total exposure duration:
72 h

Test conditions

pH:
3.46 to 7.61
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): Petri dish 14 cm diameter
- No. of seeds per container: 50
- No. of replicates per treatment group: 1
- No. of replicates per control: 1


GROWTH CONDITIONS
- Photoperiod: no direct sunlight

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
root length after 72 hours

VEHICLE CONTROL PERFORMED: no

TEST CONCENTRATIONS
- Spacing factor for test concentrations: < 2
- Test concentrations: 25, 50, 100, 200. 220, 220, 240, 260, 280, 300 and 400 mg Sn/L
Nominal and measured concentrations:
Nominal: control,25, 50, 100, 200. 220, 220, 240, 260, 280, 300 and 400 mg Sn/L
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Species:
Sinapis alba
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
281.2 other: mg/L
Nominal / measured:
nominal
Conc. based on:
element
Basis for effect:
other: root length
Remarks on result:
other: 95% C.I.: 278.4-291.3 mg Sn/L

Applicant's summary and conclusion

Conclusions:
LC50 root growth Sinapis alba (72-h): 281.2 mg Sn/L
Executive summary:

In an terrestrial plant study, the effect of tin dichloride on the root growth of Sinapis alba seeds has been determined by Fargasova (1994). The test was performed similar to OPPTS 8500.4200. The result was based on dissolved tin ions. The 72 -hour LC50 based on tin2+ is 281.1 mg/L.

 

Based on the molecular masses, the expected toxicity of tin sulfide was calculated to be 4.572 mg/L.

This is more than a factor of 5000 above the limit of water solubility of tin sulfide. Hence it can be concluded that tin sulfide will have no acute or long-term toxic effects on Chironomus plumosos.