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EC number: 215-252-9
CAS number: 1315-01-1
Table 1.a. Summary
results of Initial Bacterial Reverse Mutation Assay in the Presence of
No. of revertants/platea
Vehicle control (SW 100µL)
aValues are means of three replicates and are rounded off to the nearest whole number
bRatio of treated/vehicle control (mean revertants per plate)
cTA98, TA100, TA1535, TA1537: 2-Aminoanthracene (4µg/plate)
dWP2uvrA (pKM101): 2-Aminoanthracene (30µg/plate)
NA: Not applicable
Table 1.b. Summary
results of Initial Bacterial Reverse Mutation Assay in the Absence of
cTA98: 2-Nitrofluorene (2µg/plate)
dTA100, TA1535: Sodium azide (1µg/plate)
eTA1537: 9-Aminoacridine (50µg/plate)
fWP2uvrA (pKM101): 4-Nitroquinoline-1-oxide (4µg/plate)
Table 2.a. Summary
results of Confirmatory Bacterial Reverse Mutation Assay in the Presence
of Metabolic Activation
Table 2.b. Summary
results of Confirmatory Bacterial Reverse Mutation Assay in the Absence
of Metabolic Activation
The test item, Tin disulfide
(CAS 1315-01-1) was tested for its mutagenic potential in the bacterial
reverse mutation assay. The study was conducted using TA98, TA100,
TA1535 and TA1537 strains of Salmonella typhimurium and WP2uvrA
(pKM101) strain of Escherichia coli. The study consisted of a
preliminary toxicity test and two mutation assays comprising four
independent experiments. The bacterial tester strains were exposed to
the test item in the presence and absence of metabolic activation system
(S-9 fraction prepared from Aroclor 1254 induced rat liver). Tin
disulfide formed a suspension in sterile water (SW) at 50 mg/mL and was
found to be homogeneous and stable up to 24 hours at 1 and 200 mg/mL
when stored at room temperature.
In a preliminary toxicity test, the mean number of revertant colonies
was more or less comparable to the SW control plates up to the highest
tested dose of 5000 µg/plate, both in the presence and absence of
metabolic activation. No toxicity of the test item was seen as the
intensity of the bacterial background lawn was comparable to that of the
SW control plates up to 5000 µg/plate, both in the presence and absence
of metabolic activation. There was a slight precipitation of the test
item on the basal agar plates at 5000 µg/plate both in the presence and
absence of metabolic activation. Based on these observations, it was
decided to test up to the maximum dose of 5000 µg/plate in the initial
as well as the confirmatory mutation assay.
In the initial mutation assay, the test item was exposed in triplicate
to 50, 158, 500, 1580 and 5000 µg/plate test doses in the presence and
absence of metabolic activation using direct plate incorporation
procedure. In the confirmatory assay, the test item was exposed in
triplicate to concentrations of 100, 266, 707, 1880 and 5000 µg/plate in
the presence and absence of metabolic activation using pre-incubation
procedure. The vehicle control (SW) and the appropriate positive
controls were tested simultaneously. The mean and standard deviation of
numbers of revertant colonies were calculated for each test dose and the
controls for all the tester strains.
The results from the initial as well as from the confirmatory
assays,indicate the tested doses showed no positive mutagenic increase
in the mean numbers of revertant colonies for all tester strains when
compared to the respective vehicle control plates, either in the
presence or absence of metabolic activation.The mean numbers of
revertant colonies neither doubled for strains TA98, TA100 and WP2uvrA
(pKM101) nor tripled for strains TA1535 and TA1537 when compared to the
respective vehicle control plates, either in the presence or in the
absence of the metabolic activation at any of the tested doses.
Under identical test conditions, there was a more than 3-fold increase
in the mean numbers of revertant colonies in the positive controls,
demonstrating the sensitivity of the assay procedure used.
The study indicated that Tin disulfide (CAS 1315-01-1) was not mutagenic
in this Bacterial Reverse Mutation Assay up to the highest tested dose
of 5000 µg/plate.
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