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EC number: 215-252-9
CAS number: 1315-01-1
disulfide had no sensitising effect in the murine local lymph node assay
according to OECD guideline 429. This was confirmed by a study on the
read-across substance tin sulfide.
key Local Lymph Node Assay (LLNA) with Tin disulfide was conducted to
study potential contact sensitisation by measuring lymphocyte
proliferative response from auricular lymph nodes following topical
application of the test item to the female CBA/Ca mouse ear (Sulaiman,
2012). After a screening phase, five female mice/group received the
vehicle (DMSO) or 30% α-hexylcinnamaldehyde (HCA: positive control in
DMSO) or 5, 10, 15, 30 and 50% w/v of Tin disulfide in DMSO on days 1 to
3. On day 6, a volume of 250 µL (20 µCi) of 3H-TdR
in PBS was administered to each mouse via the lateral tail vein, and
uptake into the auricular lymph nodes draining the site of test item
application was measured five hours post administration. Proper conduct
of the LLNA was confirmed via a positive response using 30% HCA, which
elicited proliferation with a Stimulation Index (SI) value of 6.10, in
comparison to vehicle-treated mice.The test item did not elicit
erythema, no clinical signs and no effect on body weight gain at the
tested concentrations. The test item at dose concentrations of 5, 10,
15, 30 and 50% w/v elicited proliferative response with SI of 0.42,
0.41, 1.98, 2.35 and 2.87, respectively in comparison with the
vehicle-treated mice. Tin disulfide therefore did not demonstrate dermal
sensitization potential in the mouse LLNA, as the lymph nodes draining
the area of topical application did not elicit a proliferative response
greater than the 3X threshold.
supporting Local Lymph Node Assay (LLNA) with Tin sulfide as read across
substance (Prochazkova, 2010) was also negative for skin sensitisation.
Tin disulfide and Tin sulfide showed a comparable toxicological profile
for this endpoint.
description of key information: Both
a key Local Lymph Node Assay (LLNA) with Tin disulfide and a supporting
LLNA with Tin sulfide were negative, indicating absence of potential for
for selection of skin sensitisation endpoint: Key
in vivo study with reference substance confirming absence of
classification and labelling is needed for skin sensitisation for Tin
disulfide according to EU labelling regulations Commission Directive
93/21/EEC and CLP regulation (No. 1272/2008 of 16 December 2008).
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