3-chloroaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

publication

Title:

#45: Toxicity of green algae and aquatic algae

Author:

MITI

Year:

2000

Bibliographic source:

http://www.safe.nite.go.jp/jcheck/

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

(1984)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-chloroaniline

EC Number:

203-581-0

EC Name:

3-chloroaniline

Cas Number:

108-42-9

Molecular formula:

C6H6ClN

IUPAC Name:

3-chloroaniline

Test material form:

other: liquid

Details on test material:

- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Scenedesmus capricornutum

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

23 ± 2 °C

Nominal and measured concentrations:

Concentrations: 0.10, 0.32, 1.0, 3.2, 10, 32 and 100 mg/L

Details on test conditions:

- Test volume: 100 mL OECD nedium per container
- Stations: 3 container per concentration
- Initial cell concentration: 1E+04 cell/mL
- Temperature: 23 ± 2 °C
- Photoperiod: 4000 ~ 5000 lux (Continuous illumination of the piston in the vicinity of the liquid surface)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

other: EbC50

Effect conc.:

9.99 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: (8.72 ~ 11.3 mg/L)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

other: NOECb

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

21.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: (19.2 ~ 23.0 mg/L)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

other: NOECr

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The measured concentrations confirmed that deviation from the nominal concentrations was less than 20 %.

Conclusions:

The acute toxicity to algae (Selenastrum capricornutum) of 3-chloroaniline was exposued for a period of 72 hours under static conditions. Inhibition of the algal population was measured as reduction in growth rate (index r) and showed an ErC50 of 21.1 mg/L ; a NOECr of 10.0 mg/L was calculated.

Executive summary:

The acute toxicity to algae (Selenastrum capricornutum) of 3-chloroaniline was exposued for a period of 72 hours under static conditions. Inhibition of the algal population was measured as reduction in growth rate (index r) and showed an ErC50 of 21.1 mg/L ; a NOECr of 10.0 mg/L was calculated.