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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
#45: Toxicity of green algae and aquatic algae
Author:
MITI
Year:
2000
Bibliographic source:
http://www.safe.nite.go.jp/jcheck/
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
(1984)
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Scenedesmus capricornutum

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 2 °C
Nominal and measured concentrations:
Concentrations: 0.10, 0.32, 1.0, 3.2, 10, 32 and 100 mg/L
Details on test conditions:
- Test volume: 100 mL OECD nedium per container
- Stations: 3 container per concentration
- Initial cell concentration: 1E+04 cell/mL
- Temperature: 23 ± 2 °C
- Photoperiod: 4000 ~ 5000 lux (Continuous illumination of the piston in the vicinity of the liquid surface)

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
9.99 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: (8.72 ~ 11.3 mg/L)
Duration:
72 h
Dose descriptor:
other: NOECb
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
21.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: (19.2 ~ 23.0 mg/L)
Duration:
72 h
Dose descriptor:
other: NOECr
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The measured concentrations confirmed that deviation from the nominal concentrations was less than 20 %.
Conclusions:
The acute toxicity to algae (Selenastrum capricornutum) of 3-chloroaniline was exposued for a period of 72 hours under static conditions. Inhibition of the algal population was measured as reduction in growth rate (index r) and showed an ErC50 of 21.1 mg/L ; a NOECr of 10.0 mg/L was calculated.
Executive summary:

The acute toxicity to algae (Selenastrum capricornutum) of 3-chloroaniline was exposued for a period of 72 hours under static conditions. Inhibition of the algal population was measured as reduction in growth rate (index r) and showed an ErC50 of 21.1 mg/L ; a NOECr of 10.0 mg/L was calculated.