Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
#44: Long-term toxicity to aquatic invertebrates (Daphnia magna)
Author:
MITI
Year:
2000
Bibliographic source:
http://www.safe.nite.go.jp/jcheck/
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Test temperature:
20 ± 1 °C
Nominal and measured concentrations:
Concentrations: 0.0032, 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L
Details on test conditions:
- Test volume: 80 mL per container
- Stations: 10 container per test concentration
- Number of animals: 10 animals per test concentration (1 animal per container)
- Temperature: 20 ± 1 °C
- Photoperiod: 16 h light / 8 h dark
- Test typ: semi-static, water change every 3 days

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: (0.10 ~ 0.25 mg/L)
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.012 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: (0.007 ~ 0.019 mg/L)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.003 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The measured concentrations confirmed that deviation from the nominal concentrations was less than 20 %.
Conclusions:
The long-term toxicity of aquatic intvertebrates (Daphnia magna) of 3-chloroaniline was determined under semi-static conditions. After 21 days exposure period a LC50 of 0.16 mg/L and an EC50 og 0.12 mg/L were measured, and a NOEC of 0.0032 mg/L and a LOEC of 0.010 mg/L was calculated.
Executive summary:

The long-term toxicity of aquatic intvertebrates (Daphnia magna) of 3-chloroaniline was determined under semi-static conditions. After 21 days exposure period a LC50 of 0.16 mg/L and an EC50 og 0.12 mg/L were measured, and a NOEC of 0.0032 mg/L and a LOEC of 0.010 mg/L was calculated.