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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1989-08-21 till 1989-12-04
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with no or acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
#28: Biodegradation in water: screening tests
Author:
MITI
Year:
1989
Bibliographic source:
http://www.safe.nite.go.jp/jcheck/
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: JIS K 0102-1986, "Test methods for industrial water"
Deviations:
not specified
Principles of method if other than guideline:
Determination of BOD and measurement of TOC.
Analytical HPLC analysis.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity: 99 % ± 1 %
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of inoculum/activated sludge:
Fushiko Fluss Behandlungsanlage (Sapporo)
Nakahama Behandlungsanlage (Osaka)
Kitakami (Ishinomaki City, Präfektur Miyagi)
Yoshino River (Tokushima)
Hiroshima Bay (Hiroshima)
Fukashiba Behandlungsanlage (Ibaraki Kashima-gun)
Ochiai Behandlungsanlage (Shinjuku-ku, Tokyo)
Shinano River (Niigata Nishikanbara County)
Biwa-See (Otsu, Shiga Präfektur)
Dokai (Kitakyushu)
- Storage conditions: dark room
- Date of colletion: June 1989
- Preparation of inoculum for exposure:
Were collected from 500 locations in Japan old and new mud. 5 L and 10 L of the old de new activated sludge were mixed and then filtered. The pH was adjusted to 7.0 + / - 1.0 set.

- Concentration of sludge: 30 mg/L suspended solids
- Concentration of suspended solids in the activated sludge: 6800 mg / L

Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
A solution of 100 mg/L 3-chloroaniline in a mineral medium was stirred in a closed flask (300 mL) and inoculated at a constant temperature (25 ± 1 °C) for up to 28 days under aerobic conditions in the dark.
The endogenous activity of the inoculum was checked running parallel blanks with inoculum but without 3-chloroaniline. A reference compound (aniline) was run in parallel to check the operation of the procedures.
A toxicity control (3-chloroaniline and reference compound) was run parallel, to ensure that the chosen concentration of 3-chloroaniline was not inhibitory to microorganisms.
Degradation was followed by the determination of oxygen uptake and measurement were taken at frequent intervals to allow the identification of the beginning and end of biodegradation.
The oxygen uptake was calculated from the readings taken at regular frequent intervals, using the method given by the manufacturer of the equipment. At the beginning and the end of incubatio, the pH was measured in the flasks.

Exposure condition:
- Test volume: 300 mL
- Test apparatus: Wakosil-5C18
- 28 days incubation at 25 ± 1 °C
Reference substance
Reference substance:
aniline
Remarks:
(100 mg/L)

Results and discussion

% Degradationopen allclose all
Parameter:
other: TOC
Value:
3
Sampling time:
28 d
Parameter:
other: TOC
Value:
2
Sampling time:
28 d
Parameter:
other: TOC
Value:
4
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
A degradation of 65 % of the reference compound was measured after 7 and 14 days.

Any other information on results incl. tables

From the beginning to the end the pH values of different flasks are in the range og 7.0 to 7.3.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Within 28 days , a degradation rate of 3 % was determined. 3-chloroaniline is considered to be "Not Readily Biodegradable". The refence compound aniline showed 65 %.
Executive summary:

Within 28 days , a degradation rate of 3 % was determined. 3-chloroaniline is considered to be "Not Readily Biodegradable". The refence compound aniline showed 65 %.