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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Study period:
2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
#41: Short-term toxicity to fish
Author:
MITI
Year:
2000
Bibliographic source:
http://www.safe.nite.go.jp/jcheck/
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18 and 32 mg/L plus control

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Oryzias latipes

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Nominal and measured concentrations:
Concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18 and 32 mg/L plus control
Details on test conditions:
- Test volume: 3 L / Container
- Number of stations: 1 container / concentration group
- Number of test organisms: 10 fish / concentration group
- Temperature: 24 ± 1 ℃
- Lighting: 16 h light / 8 h dark
- Analysis: HPLC
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8.79 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 6.66 ~ 11.63 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The acute toxicity to fish (Oryzias latipes) was tested under semi-static conditions. After 96 hours of exposure a LC50 of 8.79 mg/L was observed.
Executive summary:

The acute toxicity to fish (Oryzias latipes) was tested under semi-static conditions. The study was concucted in accordance to the OECD guideline 203 "Fish, Acute Toxicity Test" (1992). The following nominal concentrations of 3-chloroaniline were tested: 1.0, 1.8, 3.2, 5.6, 10, 18 and 32 mg/L. After 96 hours of exposure a LC50 of 8.79 mg/L was determined.