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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
other information
Rationale for reliability incl. deficiencies:
other: Reliable with restrictions.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Uptake, Elimination, and Bioconcentration of ten Anilines in Zebrafish (Brachydanio rerio)C
Author:
Kalsch, W. et al.
Year:
1991
Bibliographic source:
Chemosphere. Vol.22. Nos 3-4, pp. 351-363, 1991
Reference Type:
publication
Title:
Comparison of BCF Models based on log P
Author:
Devillers, J. et al.
Year:
1996
Bibliographic source:
Chemosphere, Vol. 33, No. 6, pp. 1047-1065, 1996

Materials and methods

GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Details on sampling:
Exposure was performed under static conditions in a closed basin with 60 fishes and 5000 mL of carbon filtered tap water, (Hardness 230 ± 20 mg/L as CaO, pH 8.1 ± 0.1 at 26 ± 1 °C). 100 mg/L streptomycine sulfate were added. Fishes were not fed during exposure. The water was aerated and waste was passed through toluene to detect evaporated anilines and through KOH solution (10 % w/v) to collect 14CO2. Water, toluene, and KOH samples were taken several times during exposure and examined for radioactivity. Activity in water was kept constant (± 10%) during exposure. Ten samples at three fishes each were taken to examine the kinetics of uptake. Steady state was considered to be reached when activity was constant in at least three samples. The remaining 30 fishes were then transferrecl to the elimination basin, where tap water was passed through (10 L/h for the first ten hours, 2 L/h thereafter). Sampies taken during elimination (three fishes each) were treated in the same way as decribed above.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Adult male artd female zebrafish (Brachydanio rerio) from West Aquarium, Bad Lauterberg, Gemany were used.
Their wet weight ranged from 150 to 450 mg.
3-chloroaniline tested was rin-14C labelled compound

Study design

Route of exposure:
aqueous
Test type:
static
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
h
Total depuration duration:
52 h

Test conditions

Hardness:
23 °dH (230 ± 20 mg/L CaO)
Test temperature:
26 ± 1 °C
pH:
8.1 ± 0.1
Nominal and measured concentrations:
Concentration of 3-chloroaniline in water during exposure: 0.17 µmol/L = 0.022 mg/L
Concentration (activity) in fish at the end of the elimination period: 10.3 %
Details on estimation of bioconcentration:


























(2)

Results and discussion

Bioaccumulation factor
Type:
BCF
Value:
11.5 dimensionless
Basis:
other: estimation
Time of plateau:
100 h
Calculation basis:
steady state

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The bioaccumulation of 3-chloroaniline was calculated by 11.5.
Executive summary:

The bioaccumulation of 3-chloroaniline was calculated by 11.5.