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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Relaible source.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
For the adsorption tests, live concentrations of the test substance in 0.01 M CaCl2 were used, ranging from 1.0 to 100 mg/L for 3-chloroaniline.
Because 3-chloroaniline have a low solubility in water, a solvent had to be added to the test solutions: methanol (max. 1 mL/L was used.

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- KOBG is a sandy soil (pH 1N KCI 4.8; 3.7 % organic matter), and was chosen as a representative of natural soils. It was collected from the top 20 cm layer of agricultural field plots.
- OECD (pH 1N KCI 5.9; 8.1 % organic matter) is an artificial soil, chosen because of its international use in earthworm toxicity tests of new and existing chemieals (OECD, 1984; EEC, 1985). OECD was made up in the Iabaratory and is a homogeneaus mixture of 10 % peat (2 mm mesh-sieved), 20 % kaolin clay, c. 69.5 % quartz sand and c. 0.5 % CaCO3.

Test organisms

Test organisms (species):
Lumbricus rubellus
Details on test organisms:
Second Species: Eisenia andrei

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Total exposure duration:
14 d

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
59.8 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Lumbricus rubellus;Soil: KOBG
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
49.5 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Lumbricus rubellus; Soil: OECD
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
72.5 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Eisenia andrei; Soil: KORG
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
92.5 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Eisenia andrei; Soil: OECD

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The acute toxicity to soil macroorganisms except arthropods were determined with two species (Lumbricus rubellus and Eisenia andrei) and two soil types (KOBG and OECD). 3-chloroaniline showed a toxicity to earthworms after 14 days a LC50 of 49.5 - 92.5 mg/L.
Executive summary:

The acute toxicity to soil macroorganisms except arthropods were determined with two species (Lumbricus rubellus and Eisenia andrei) and two soil types (KOBG and OECD). 3-chloroaniline showed a toxicity to earthworms after 14 days a LC50 of 49.5 - 92.5 mg/L.