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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
publication
Title:
Acute toxicological evaluation of acrylonitrile (1969)
Author:
Vernon PA, Dulak LH & Deskin R
Year:
1990
Bibliographic source:
Journal of American College of Toxicology (1990):1, 114-115

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
yes
Remarks:
: scores for erythem and oedema combhined; intact and abraded skin sites used; 24-hour exposure
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rabbit
Strain:
New Zealand White

Test system

Type of coverage:
occlusive
Preparation of test site:
other: sites were shorn, intact and abraded sites were used
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
0.5 ml
Duration of treatment / exposure:
24 hours
Observation period:
72 hours
Number of animals:
Six

Results and discussion

In vivo

Results
Irritation parameter:
other: combined mean score for oedema and erythema
Basis:
mean
Time point:
other: 24-72h
Score:
3.6
Reversibility:
not fully reversible within: 72h

Any other information on results incl. tables

The mean score both erythema and oedema was 3.6, with slightly higher scores reported for abraded skin.

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The results of this study indicate that acrylonitrile is a skin irritant, but is not corrosive.
Executive summary:

In this guideline-comparable study, 0.5 mL acrylonitrile was applied for 24 hours under occlusive conditions to the shorn (intact and abraded) dorsal skin of six New Zealand White rabbits. Dermal reactions were assessed at 24 and 72 hours following application and mean scores (24 and 72 hour) scores (on a scale of 0 to 4) for both erythema and oedema are reported. The mean score for both erythema and oedema was 3.6, with slightly higher scores reported for abraded skin. This study that acrylonitrile is a skin irritant and should be classified as such.