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EC number: 203-466-5 | CAS number: 107-13-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 21 September 1987 - 17 March 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: Biodegradation test of a chemical substance using a microorganism etc." prescribed in "Test Method Concerning New Chemical Substances" (Notification date 13 July 1974, Chemical Substances Control Law, Japan).
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Ten different sources in Japan, such as sewage-treatment plants, rivers, lakes and seas.
- Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD
- Parameter followed for biodegradation estimation:
- TOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- GC
- Details on study design:
- TEST CONDITIONS
- Test temperature: 25±1˚C
- pH: No data
- pH adjusted: No data
TEST SYSTEM
- Number of culture flasks/concentration:
3 bottles (test substance + sludge)
1 bottle (test substance + water)
1 bottle (reference substance + sludge)
- Measuring equipment: continuous BOD meter
SAMPLING
- Sampling frequency: continuous for BOD; After 28 days for TOC and GC
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 bottle was set - Reference substance:
- aniline
- Remarks:
- 100 mg/L
- Parameter:
- other: BOD(NO2)
- Value:
- 15
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean value
- Parameter:
- other: BOD(NH3)
- Value:
- 23
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean value
- Parameter:
- % degradation (TOC removal)
- Value:
- 38
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean value
- Parameter:
- other: GC
- Value:
- 44
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean value
- Details on results:
- - Biodegradabily by BOD(NO2) after 28 days: 24 %, 5 % and 15 % (mean: 15 %)
- Biodegradabily by BOD(NH3) after 28 days: 37 %, 8 % and 24 % (mean: 23 %)
- Biodegradabily by TOC after 28 days: 54 %, 25 % and 35 % (mean: 38 %)
- Biodegradabily by GC after 28 days: 61 %, 29 % and 41 % (mean: 44%)
- Kinetics of test substance(BOD (NO2)):
0, 0, 0 % (7th day)
1, 0, 0 % (14th day)
24, 5, 15 % (28th day)
- Kinetics of test substance (BOD (NH3)):
0, 0, 0 % (7th day)
2, 0, 0 % (14th day)
37, 8, 24 % (28th day)
- Degradation products: No - Results with reference substance:
- - Kinetics of control substance (BOD (NO2)):
78 % (7th day), 96 % (14th day)
- Kinetics of control substance (BOD (NH3)):
78 % (7th day), 96 % (14th day) - Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively.
The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively. - Executive summary:
The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively.
The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: 'Biodegradation test of a chemical substance using a microorganism etc.' prescribed in 'Test Method Concerning New Chemical Substances' (Notification date 13 July 1974. Chemical Substances Control Law, Japan)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Ten different sources in Japan, such as sewage-treatment plants, rivers, lakes and seas.
- Concentration of sludge: 100 mg/L - Duration of test (contact time):
- 14 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD
- Parameter followed for biodegradation estimation:
- TOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- GC analysis
- Details on study design:
- TEST CONDITIONS
- Test temperature: 25±1˚C
- pH: No data
- pH adjusted: No data
TEST SYSTEM
- Number of culture flasks/concentration:
3 bottles (test substance + sludge)
2 bottles (test substance + water)
1 bottle (reference substance + sludge)
- Measuring equipment: continuous BOD meter
SAMPLING
- Sampling frequency: continuous for BOD; After 14 days for TOC and GC
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles were set
- Water blank: 1 bottle was set - Reference substance:
- aniline
- Remarks:
- 100 mg/L
- Preliminary study:
- No preliminary study
- Test performance:
- No information
- Parameter:
- other: BOD(NO2)
- Value:
- 61
- Sampling time:
- 14 d
- Remarks on result:
- other: Mean value
- Parameter:
- other: BOD(NH3)
- Value:
- 96
- Sampling time:
- 14 d
- Remarks on result:
- other: Mean value
- Parameter:
- % degradation (TOC removal)
- Value:
- 100
- Sampling time:
- 14 d
- Remarks on result:
- other: mean value
- Parameter:
- other: GC
- Value:
- 100
- Sampling time:
- 14 d
- Details on results:
- - Biodegradabily by BOD(NO2) after 14 days: 74 %, 67 % and 41 % (mean: 61 %)
- Biodegradabily by BOD(NH3) after 14 days: 117 %, 107 % and 65 % (mean: 96 %)
- Biodegradabily by TOC after 14 days: 100 %, 100 % and 100 % (mean: 100 %)
- Biodegradabily by GC after 14 days: 100 %, 100 % and 100 %
- Kinetics of test substance(BOD (NO2)):
46, 21, 10 % (7th day)
74, 67, 41 % (14th day)
- Kinetics of test substance (BOD (NH3)):
72, 34, 15 % (7th day)
117, 107, 65 % (14th day)
- Degradation products: No - Results with reference substance:
- - Kinetics of control substance (BOD (NO2)):
64 % (7th day), 86 % (14th day)
- Kinetics of control substance (BOD (NH3)):
64 % (7th day), 86 % (14th day) - Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively.
The determination by the both TOC and GC methods showed 100% degradation of the test substance. - Executive summary:
The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively. The determination by the both TOC and GC methods showed 100% degradation of the test substance.
Referenceopen allclose all
The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively. The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively. |
The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively. The determination by the both TOC and GC methods showed 100% degradation of the test substance. |
Description of key information
The results of a study performed to OECD301C (CITI, 1988) indicate that acrylonitrile was not readily biodegradable under the test conditions. In contrast, the results of a study performed to OECD 302C (CITI, 1988) indicate that acrylonitrile was inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
The results of a study performed to OECD301C (CITI, 1988) indicate that acrylonitrile was not readily biodegradable under the test conditions. In contrast, the results of a study performed to OECD302C (CITI, 1988) indicate that acrylonitrile was inherently biodegradable.
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