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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
21 September 1987 - 17 March 1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: Biodegradation test of a chemical substance using a microorganism etc." prescribed in "Test Method Concerning New Chemical Substances" (Notification date 13 July 1974, Chemical Substances Control Law, Japan).
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Ten different sources in Japan, such as sewage-treatment plants, rivers, lakes and seas.
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
GC
Details on study design:
TEST CONDITIONS
- Test temperature: 25±1˚C
- pH: No data
- pH adjusted: No data

TEST SYSTEM
- Number of culture flasks/concentration:
3 bottles (test substance + sludge)
1 bottle (test substance + water)
1 bottle (reference substance + sludge)
- Measuring equipment: continuous BOD meter


SAMPLING
- Sampling frequency: continuous for BOD; After 28 days for TOC and GC


CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 bottle was set
Reference substance:
aniline
Remarks:
100 mg/L
Parameter:
other: BOD(NO2)
Value:
15
Sampling time:
28 d
Remarks on result:
other: Mean value
Parameter:
other: BOD(NH3)
Value:
23
Sampling time:
28 d
Remarks on result:
other: Mean value
Parameter:
% degradation (TOC removal)
Value:
38
Sampling time:
28 d
Remarks on result:
other: Mean value
Parameter:
other: GC
Value:
44
Sampling time:
28 d
Remarks on result:
other: Mean value
Details on results:
- Biodegradabily by BOD(NO2) after 28 days: 24 %, 5 % and 15 % (mean: 15 %)
- Biodegradabily by BOD(NH3) after 28 days: 37 %, 8 % and 24 % (mean: 23 %)
- Biodegradabily by TOC after 28 days: 54 %, 25 % and 35 % (mean: 38 %)
- Biodegradabily by GC after 28 days: 61 %, 29 % and 41 % (mean: 44%)

- Kinetics of test substance(BOD (NO2)):
0, 0, 0 % (7th day)
1, 0, 0 % (14th day)
24, 5, 15 % (28th day)

- Kinetics of test substance (BOD (NH3)):
0, 0, 0 % (7th day)
2, 0, 0 % (14th day)
37, 8, 24 % (28th day)

- Degradation products: No
Results with reference substance:
- Kinetics of control substance (BOD (NO2)):
78 % (7th day), 96 % (14th day)
- Kinetics of control substance (BOD (NH3)):
78 % (7th day), 96 % (14th day)

The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively.

The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively.

Interpretation of results:
not readily biodegradable
Conclusions:
The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively.
The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively.
Executive summary:

The test substance is not readily biodegradable under the test condition since the degradations after 28days were 15% and 23% related to BOD(NO2) and BOD(NH3), respectively.

The determination by the TOC and GC methods showed 38% and 44% degradation of the test substance, respectively.

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: 'Biodegradation test of a chemical substance using a microorganism etc.' prescribed in 'Test Method Concerning New Chemical Substances' (Notification date 13 July 1974. Chemical Substances Control Law, Japan)
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Ten different sources in Japan, such as sewage-treatment plants, rivers, lakes and seas.
- Concentration of sludge: 100 mg/L
Duration of test (contact time):
14 d
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
GC analysis
Details on study design:
TEST CONDITIONS
- Test temperature: 25±1˚C
- pH: No data
- pH adjusted: No data


TEST SYSTEM
- Number of culture flasks/concentration:
3 bottles (test substance + sludge)
2 bottles (test substance + water)
1 bottle (reference substance + sludge)
- Measuring equipment: continuous BOD meter


SAMPLING
- Sampling frequency: continuous for BOD; After 14 days for TOC and GC


CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles were set
- Water blank: 1 bottle was set
Reference substance:
aniline
Remarks:
100 mg/L
Preliminary study:
No preliminary study
Test performance:
No information
Parameter:
other: BOD(NO2)
Value:
61
Sampling time:
14 d
Remarks on result:
other: Mean value
Parameter:
other: BOD(NH3)
Value:
96
Sampling time:
14 d
Remarks on result:
other: Mean value
Parameter:
% degradation (TOC removal)
Value:
100
Sampling time:
14 d
Remarks on result:
other: mean value
Parameter:
other: GC
Value:
100
Sampling time:
14 d
Details on results:
- Biodegradabily by BOD(NO2) after 14 days: 74 %, 67 % and 41 % (mean: 61 %)
- Biodegradabily by BOD(NH3) after 14 days: 117 %, 107 % and 65 % (mean: 96 %)
- Biodegradabily by TOC after 14 days: 100 %, 100 % and 100 % (mean: 100 %)
- Biodegradabily by GC after 14 days: 100 %, 100 % and 100 %

- Kinetics of test substance(BOD (NO2)):
46, 21, 10 % (7th day)
74, 67, 41 % (14th day)

- Kinetics of test substance (BOD (NH3)):
72, 34, 15 % (7th day)
117, 107, 65 % (14th day)

- Degradation products: No
Results with reference substance:
- Kinetics of control substance (BOD (NO2)):
64 % (7th day), 86 % (14th day)
- Kinetics of control substance (BOD (NH3)):
64 % (7th day), 86 % (14th day)

The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively.

The determination by the both TOC and GC methods showed 100% degradation of the test substance.

Interpretation of results:
inherently biodegradable
Conclusions:
The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively.

The determination by the both TOC and GC methods showed 100% degradation of the test substance.
Executive summary:

The test substance is inherently biodegradable under the test condition since the degradations after 14days were 61% and 96% related to BOD(NO2) and BOD(NH3), respectively. The determination by the both TOC and GC methods showed 100% degradation of the test substance.

Description of key information

The results of a study performed to OECD301C (CITI, 1988) indicate that acrylonitrile was not readily biodegradable under the test conditions.  In contrast, the results of a study performed to OECD 302C (CITI, 1988) indicate that acrylonitrile was inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information

The results of a study performed to OECD301C (CITI, 1988) indicate that acrylonitrile was not readily biodegradable under the test conditions. In contrast, the results of a study performed to OECD302C (CITI, 1988) indicate that acrylonitrile was inherently biodegradable.