3,4-dichloroaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: peer reviewed data cited in EU risk assessment report comparable to guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Consecutive system of tests for assessment of the effects of chemical agents in the aquatic environment

Author:

Adema D.M.M., Kuiper J., Hanstveit A.O., Canton H.H.

Year:

1982

Bibliographic source:

Pestic. Chem. 3, 537-544

Reference 2

Reference Type:

review article or handbook

Title:

2,4-Dichloranilin; 2,5-Dichloranilin; 3,4-Dichloranilin

Author:

Beratergremium für umweltrelevante Altstoffe (BUA)

Year:

1994

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft, BUA report 140

Reference 3

Reference Type:

review article or handbook

Title:

European Union Risk Assessment Report: 3,4-dichloraniline (3,4-DCA), Vol. 65

Author:

European Chemicals Bureau

Year:

2006

Bibliographic source:

EU RAR: 3,4-dichloraniline (3,4-DCA), Vol. 65; EUR 22235 EN ISSN 1018-5593

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichloroaniline

EC Number:

202-448-4

EC Name:

3,4-dichloroaniline

Cas Number:

95-76-1

Molecular formula:

C6H5Cl2N

IUPAC Name:

3,4-dichloroaniline

Details on test material:

purity: no data

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Scenedesmus pannonicus

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

20 °C

Details on test conditions:

constant light

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Basis for effect:

growth rate

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

4.8 mg/L

Basis for effect:

growth rate

Applicant's summary and conclusion

Executive summary:

Adema et al (1982), Pestic. Chem. 3, 537 -455

The EC50 (96 h) for growth inhibition of the freshwater algae Scenedesmus pannonicus determined according to OECD Guideline 201 was determined to be 4.8 mg/L. Furthermore, the NOEC was determined to be 1 mg/L.