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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report Date:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton Buchanan
- Source: Bio International B.V. (nederlands)
- Weight at study initiation: 1.0-3.0 cm



FEEDING DURING TEST
- Food type: Tetra Min fish food
- Amount: 2% fish weight/day
- Frequency: 3 times a day

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d

Test conditions

Hardness:
14-14.3 °dH
Test temperature:
22 +- 1°C
pH:
ca. 8 +- 1 °C
Dissolved oxygen:
ca 90-100 % saturation
Nominal and measured concentrations:
Nominal concentrations: 0.05, 0.16, 0.5, 1.6, 5.0 mg/L. Additionally, a control was tested
Details on test conditions:
TEST SYSTEM
- Test vessel:
- size, fill volume: 300*135*200 mm, 5 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): 3 times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 light dark cycle



EFFECT PARAMETERS MEASURED : behaviour and mortality

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1.18 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LOEC
Effect conc.:
3.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion