3,4-dichloroaniline

Long-term toxicity to fish

Administrative data

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichloroaniline

EC Number:

202-448-4

EC Name:

3,4-dichloroaniline

Cas Number:

95-76-1

Molecular formula:

C6H5Cl2N

IUPAC Name:

3,4-dichloroaniline

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton Buchanan
- Source: Bio International B.V. (nederlands)
- Weight at study initiation: 1.0-3.0 cm



FEEDING DURING TEST
- Food type: Tetra Min fish food
- Amount: 2% fish weight/day
- Frequency: 3 times a day

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Test conditions

Hardness:

14-14.3 °dH

Test temperature:

22 +- 1°C

pH:

ca. 8 +- 1 °C

Dissolved oxygen:

ca 90-100 % saturation

Nominal and measured concentrations:

Nominal concentrations: 0.05, 0.16, 0.5, 1.6, 5.0 mg/L. Additionally, a control was tested

Details on test conditions:

TEST SYSTEM
- Test vessel:
- size, fill volume: 300*135*200 mm, 5 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): 3 times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 light dark cycle



EFFECT PARAMETERS MEASURED : behaviour and mortality

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1.18 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LOEC

Effect conc.:

3.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality