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EC number: 202-448-4 | CAS number: 95-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- European Union Risk Assessment Report 3,4 -dichloroaniline (3,4 -DCA)
- Author:
- European Chemicals Bureau
- Year:
- 2 006
- Bibliographic source:
- EU Risk Assesment Report , Vol. 65 (2006)
- Reference Type:
- publication
- Title:
- A comparative study of the toxicity of 1,1,2-trichloroethane, Dieldrin, Pentachlorophenol and 3,4-dichloroaniline for marine and fresh water organisms
- Author:
- Adema DMM, Vink GJ, 533-554
- Year:
- 1 981
- Bibliographic source:
- Chemosphere Vol. 10, No. 6, 533-554
Materials and methods
- Principles of method if other than guideline:
- TNO (1980): Degradability, Ecotoxicity and Bioaccumulation; The determination of the possible effects of chemicals and wastes on the aquatic environment. Government Publishing Office, The Hague, The Netherlands.
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,4-dichloroaniline
- EC Number:
- 202-448-4
- EC Name:
- 3,4-dichloroaniline
- Cas Number:
- 95-76-1
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 3,4-dichloroaniline
- Details on test material:
- no data; purity not reported ("chemically pure")
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: concentration of the test compound was measured by chemical analysis during the test and was at least 70% of the nominal value
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation: life stage: larvae
- Length at study initiation: 1 mm
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- pH:
- the pH was measured during the test and was always about 8
- Dissolved oxygen:
- the oxygen concentration was measured during the test and was never less than 70% of the saturation value
- Nominal and measured concentrations:
- measured concentration was at least 70% of the nominal value
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.23 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.16 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Executive summary:
Adema, D.M.M. and Vink, G.J. (1981) / EU RAR (2006):
The acute toxicity of 3,4 -DCA was determined with an acute toxicity test with the test species Daphnia magna (larvae, size 1 mm). The test was conducted after a test method developed by the dutch ministry of transport and public works (TNO 1980). The test is based on the relationship between the constant test item concentration in the test water and the effect on the organism in the absence of other circumstances unfavourable for the test organism.
The test compound was chemically pure 3,4 -dichloroaniline.
The test met the following requirements:
- the concentration of the test compound in the water was measured by chemical analysis during the test and was at least 70% of the nominal value
- the oxygen concentration was measured during the test and was never less than 70% of the saturation level
- the mortality of the untreated daphnids was equal to or lower than that permitted in the TNO protocol
- the pH was measured during the test and was always about 8
The LC50 after 48 hours was determined with 0.23 mg/L, the moratality after 96 hours was determined with LC50 = 0.16 mg/L.
Other lethal concentration values determined in this test (larva: 24 hours, 7 days, 14 days, 3 weeks; adult: 48 hours, 96 hours, 7 days) were not adopted in the EU-Risk Assesment Report or belonged to long-term toxicity.
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