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EC number: 202-448-4
CAS number: 95-76-1
Adema, D.M.M. and Vink, G.J. (1981) / EU RAR (2006):
The acute toxicity of 3,4 -DCA was determined with an acute toxicity
test with the test species Daphnia magna (larvae, size 1 mm). The test
was conducted after a test method developed by the dutch ministry of
transport and public works (TNO 1980). The test is based on the
relationship between the constant test item concentration in the test
water and the effect on the organism in the absence of other
circumstances unfavourable for the test organism.
The test compound was chemically pure 3,4 -dichloroaniline.
The test met the following requirements:
- the concentration of the test compound in the water was measured by
chemical analysis during the test and was at least 70% of the nominal
- the oxygen concentration was measured during the test and was never
less than 70% of the saturation level
- the mortality of the untreated daphnids was equal to or lower than
that permitted in the TNO protocol
- the pH was measured during the test and was always about 8
The LC50 after 48 hours was determined with 0.23 mg/L, the moratality
after 96 hours was determined with LC50 = 0.16 mg/L.
Other lethal concentration values determined in this test (larva: 24
hours, 7 days, 14 days, 3 weeks; adult: 48 hours, 96 hours, 7 days) were
not adopted in the EU-Risk Assesment Report or belonged to long-term
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