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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
European Union Risk Assessment Report 3,4 -dichloroaniline (3,4 -DCA)
Author:
European Chemicals Bureau
Year:
2006
Bibliographic source:
EU Risk Assesment Report , Vol. 65 (2006)
Reference Type:
publication
Title:
A comparative study of the toxicity of 1,1,2-trichloroethane, Dieldrin, Pentachlorophenol and 3,4-dichloroaniline for marine and fresh water organisms
Author:
Adema DMM, Vink GJ, 533-554
Year:
1981
Bibliographic source:
Chemosphere Vol. 10, No. 6, 533-554

Materials and methods

Principles of method if other than guideline:
TNO (1980): Degradability, Ecotoxicity and Bioaccumulation; The determination of the possible effects of chemicals and wastes on the aquatic environment. Government Publishing Office, The Hague, The Netherlands.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data; purity not reported ("chemically pure")

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling method: concentration of the test compound was measured by chemical analysis during the test and was at least 70% of the nominal value

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation: life stage: larvae
- Length at study initiation: 1 mm

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

pH:
the pH was measured during the test and was always about 8
Dissolved oxygen:
the oxygen concentration was measured during the test and was never less than 70% of the saturation value
Nominal and measured concentrations:
measured concentration was at least 70% of the nominal value

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.23 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.16 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

Adema, D.M.M. and Vink, G.J. (1981) / EU RAR (2006):

The acute toxicity of 3,4 -DCA was determined with an acute toxicity test with the test species Daphnia magna (larvae, size 1 mm). The test was conducted after a test method developed by the dutch ministry of transport and public works (TNO 1980). The test is based on the relationship between the constant test item concentration in the test water and the effect on the organism in the absence of other circumstances unfavourable for the test organism.

The test compound was chemically pure 3,4 -dichloroaniline.

The test met the following requirements:

- the concentration of the test compound in the water was measured by chemical analysis during the test and was at least 70% of the nominal value

- the oxygen concentration was measured during the test and was never less than 70% of the saturation level

- the mortality of the untreated daphnids was equal to or lower than that permitted in the TNO protocol

- the pH was measured during the test and was always about 8

The LC50 after 48 hours was determined with 0.23 mg/L, the moratality after 96 hours was determined with LC50 = 0.16 mg/L.

Other lethal concentration values determined in this test (larva: 24 hours, 7 days, 14 days, 3 weeks; adult: 48 hours, 96 hours, 7 days) were not adopted in the EU-Risk Assesment Report or belonged to long-term toxicity.