3,4-dichloroaniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

European Union Risk Assessment Report 3,4 -dichloroaniline (3,4 -DCA)

Author:

European Chemicals Bureau

Year:

2006

Bibliographic source:

EU Risk Assesment Report , Vol. 65 (2006)

Reference 2

Reference Type:

publication

Title:

A comparative study of the toxicity of 1,1,2-trichloroethane, Dieldrin, Pentachlorophenol and 3,4-dichloroaniline for marine and fresh water organisms

Author:

Adema DMM, Vink GJ, 533-554

Year:

1981

Bibliographic source:

Chemosphere Vol. 10, No. 6, 533-554

Materials and methods

Principles of method if other than guideline:

TNO (1980): Degradability, Ecotoxicity and Bioaccumulation; The determination of the possible effects of chemicals and wastes on the aquatic environment. Government Publishing Office, The Hague, The Netherlands.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichloroaniline

EC Number:

202-448-4

EC Name:

3,4-dichloroaniline

Cas Number:

95-76-1

Molecular formula:

C6H5Cl2N

IUPAC Name:

3,4-dichloroaniline

Details on test material:

no data; purity not reported ("chemically pure")

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling method: concentration of the test compound was measured by chemical analysis during the test and was at least 70% of the nominal value

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation: life stage: larvae
- Length at study initiation: 1 mm

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

pH:

the pH was measured during the test and was always about 8

Dissolved oxygen:

the oxygen concentration was measured during the test and was never less than 70% of the saturation value

Nominal and measured concentrations:

measured concentration was at least 70% of the nominal value

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.23 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.16 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Executive summary:

Adema, D.M.M. and Vink, G.J. (1981) / EU RAR (2006):

The acute toxicity of 3,4 -DCA was determined with an acute toxicity test with the test species Daphnia magna (larvae, size 1 mm). The test was conducted after a test method developed by the dutch ministry of transport and public works (TNO 1980). The test is based on the relationship between the constant test item concentration in the test water and the effect on the organism in the absence of other circumstances unfavourable for the test organism.

The test compound was chemically pure 3,4 -dichloroaniline.

The test met the following requirements:

- the concentration of the test compound in the water was measured by chemical analysis during the test and was at least 70% of the nominal value

- the oxygen concentration was measured during the test and was never less than 70% of the saturation level

- the mortality of the untreated daphnids was equal to or lower than that permitted in the TNO protocol

- the pH was measured during the test and was always about 8

The LC50 after 48 hours was determined with 0.23 mg/L, the moratality after 96 hours was determined with LC50 = 0.16 mg/L.

Other lethal concentration values determined in this test (larva: 24 hours, 7 days, 14 days, 3 weeks; adult: 48 hours, 96 hours, 7 days) were not adopted in the EU-Risk Assesment Report or belonged to long-term toxicity.