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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Orally administered 3,4-dichloroaniline is absorbed by the body, metabolized in the liver and excreted mainly via urine. A low dose of 3,4-dichloroaniline (approx. 5 µg) was excreted to about 80 % within 24 h and completely excreted within 72 h by rats, without any bioaccumulation in tissues (Worobey and Shields, 1991). Oral as well as dermal administration of a higher dose of 3,4-dichloroaniline (12 mg) instead led to an increased excretion of parent compound (indicating saturation of the biotransformation pathways) and a total excretion of only 2 % of the administered dose within 24 h (El Marbouh et al., 2002). The main metabolite detected in urine of male rats was 2-amino-4,5-dichlorophenol. In vitro rat liver microsomes metabolized 3,4-dichloroaniline in the presence of NADPH-regenerating system (indicating the involvement of CYP450 -Phase I enzymes) to the two main metabolites N-hydroxy- and 6-hydroxy-3,4 -dichloroaniline with apparent km-values of 0.12 mM and 0.29 mM, respectively (McMillan, 1990a). Pretreatment of rats with 3,4-dichloraniline (100 mg/ kg, 3 d, i.p.) increased NADPH-dependent metabolite formation in rats by about two-fold (Mc Millan, 1990b). 3,4-dichloroaniline therefore is a weak inducer of microsomal enzymes. 3,4-dichloroaniline metabolism in male rabbit microsomes resembles that in rats. N-hydroxy- and 6-hydroxy-3,4-dichloroaniline are the main metabolites, with N-hydroxy-3,4-dichloroaniline being able to induce methemoglobin formation (Lenk and Sterzl, 1978). Very little dermal absorption through intact skin was observed within the first 4 h of application (Levillain, 1998). Nevertheless the choice of solvent (emulsifier) and the condition of the skin (lesion) can enhance the amount of 3,4-dichloroaniline that is absorbed through the skin (Marty, 1979).