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EC number: 202-805-4 | CAS number: 99-97-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Additional information
Short term toxicity to fish:
Based on the various studies available with Klimish rating 2 for the target substance CAS NO 99-97-8 The results is summarized as follows
Sr.No | Endpoint | Effect values | Basis for effects | Duration | Species | Remarks |
1 | TLm | 44 mg/l | mortality | 24 hrs | Oryzias latipes | Data from publication for target chemical |
2 | TLm | 20 mg/l | mortality | 24 hrs | Oryzias latipes | Data from publication for target chemical |
3 | LC50 | 52.8 mg/l | mortality | 96hrs | Pimephales promelas | Data from publication for target chemical |
4 | LC50 EC50 | 52 mg/l 52 mg/l | mortality | 96hrs | Pimephales promelas | Data from publication for target chemical |
3 | LC50 EC50 | 46 mg/l 41.5 mg/l | mortality | 96hrs | Pimephales promelas | Data from publication for target chemical |
By applying weight of evidence approach to the studies summarized in the above table it can be observed that the LC50 values was found to be in range of 46 to 52 mg/l in 96 hrs and the EC50 values was found to be in the range of 41.5 to 52 mg/l in 96 hrs. Also the Median Tolerence limit (TLm) value was found to be in the range of 20 to 44 mg/l in 24 hrs study on the basis of mortality effect. Since the substance was not found to be rapidly degradable. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that N,N-dimethyl-p-toluidine exhibit short term toxicity to fish (Oryzias latipes,Pimephales promelas) in aquatic chronic category 3.
Long term toxicity to fish:
According to column 1 of Annex IX , long-term toxicity testing on fish was considered for waiver as short term toxicity data has already been provided as part of Annex VIII requirements in section 6.1.1 of this dossier
Short term toxicity to aquatic invertebrate:
Based on the various studies available with Klimish rating 2 and 4 for target chemical CAS NO 99-97-5 and the read across substances . The results is summarized as follows
Sr.No | Endpoint | Effect values | Basis for effects | Duration | Species | Remarks |
1 | LC50 | 15.259 mg/l | mortality | 48 hrs | Daphnia magna | Predicted data for target chemical |
2 | EC50 | 23.758 mg/L.
| mortality | 48 hrs | Daphnia magna | Data from database |
3 | EC50 | 13.7 mg/L.
| mortality | 48 hrs | Daphnia magna | Data from publication for CAS no 121-69-7 |
By applying weight of evidence approach to the studies summarized in the above table, the EC50 value is expected to be in range of 13.7 to 23.758 mg/l and LC50 value was found to be 15.259 mg/l in 48 hrs study on the basis of mortality effect. Since N,N-dimethyl-p-toluidine is not found to be rapidly degradable. Thus according to CLP criteria of aquatic classification it is concluded that the substance N,N-dimethyl-p-toluidine is toxic to aquatic invertebrate (Daphnia magna)in aquatic chronic category 3
Long term toxicity to aquatic invertebrate:
According to column 1 of Annex IX , long-term toxicity testing on aquatic invertebrates was considered for waiver as short term toxicity data has already been provided as part of Annex VII requirements in section 6.1.3 of this dossier.
Toxicity to aquatic algae and cynobacteria:
Based on the various studies available with Klimish rating 2 and 4 for the target substance CAS NO 99-97-8 and read across substances and based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows
Sr.No | Endpoint | Effect values | Basis for effects | Duration | Species | Remarks |
1 | EC50 | 15.481 mg/l | growth rate | 96 hrs hrs | Green algae | Data is from database |
2 | EC50 | 24.37mg/l | Growth | 72 hrs | Pseudokirchneriella subcapitata | Predicted data of target chemcial |
3 | EC50 | 22 mg/l | growth rate | 72 hrs | Chlorella pyrenoidosa | Data from publication for CAS no 121-69-7 |
By applying weight of evidence approach to the studies summarized in the above table it can be observed that EC50 value is expected to be in range of15.481 to24.37 mg/l in 72 hrs to 96 hrs study on the basis of growth rate. Sincesubstance was not found to be rapidly degradable.Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that N,N-dimethyl-p-toluidine does exhibit toxicity to (green algae, Pseudokirchnerella subcapitata, Chlorella pyrenoidosa) in aquatic chronic category 3
Toxicity to microorganism:
Based on the various studies available with Klimish rating 2 and 4 for the target substance CAS NO 99-97-8 and read across substances and based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows
Sr.No | Endpoint | Effect values | Basis for effects | Duration | Species | Remarks |
1 | IGC50 | 80.53 mg/l | growth inhibition | 48 hrs | Tetrahymena pyriformis
| Predicted data for target chemical |
2 | EC50
| 161.2 mg/l
| growth inhibition | 24 hrs | Tetrahymena pyriformis
| Predicted data for target chemical |
3 | EC50
| 42.86 mg/l
| growth inhibition | 48 hrs | Tetrahymena pyriformis
| Predicted data for target chemical |
4 | IGC50 | 32.6 mg/l | growth inhibition | 48 hrs | Tetrahymena pyriformis
| Data from publication for CAS no 91-66-7 |
5 | EC50
| 13.6 mg/L
| reduction in light emission | 30 min | Photobacterium phosphoreum | Data from publication for CAS no 121-69-7 |
By applying weight of evidence approach to the studies summarized in the above table it can be observed that EC50 value is expected to be in the range of 13.6 to 161.2 mg/l based on the growth inhibition effect for various duration starting from 30 mins till 48 hours.
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