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EC number: 201-114-5 | CAS number: 78-40-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: the chemical was tested within the National toxicology program (NTP)
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity tests: III. Results from the testing of 255 chemicals
- Author:
- Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W
- Year:
- 1 987
- Bibliographic source:
- Environ Mol Mutagen 9, Suppl 9: 1-110
Materials and methods
- Principles of method if other than guideline:
- The preincubation method was used. The test chemical, Salmonella culture, and S-9 mix or buffer were incubated at 37°C, without shaking, for 20 min. The top agar was added. and the contents of the tubes were mixed and poured onto the surface of petri dishes. The histidine-revertant (his+) colonies arising on these plates were counted following 2 days incubation at 37°C. The plates were hand-counted when a precipitate was present; otherwise automatie colony counters were used.
All chemicals were tested initially in a toxicity assay to determine the appropriate dose range. Toxic concentrations were those at which a decrease in the number of his+ colonies was seen or at which there was a clearing in the density of the background lawn. At least five doses of the chemical were tested in triplicate. Each chemical was tested initially at half-log doses up to a dose that elicited toxicity; subsequent trials occasionally used narrower dose increments. Chemicals that were not toxic were tested to a maximum dose of 10 mg/plate. Chemicals that were poorly soluble were tested up to a dose defined by their solubility.
Concurrent solvent and positive controls were run with each trial. The positive controls in the absence of metabolie activation were sodium azide (TA1535 and TA100), 9-aminoacridine (TA97 and TAI537), and 4-nitro-o-phenylenediamine (TA98). The positive control for metabolic activation was 2-aminoanthracene for all strains. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Triethyl phosphate
- EC Number:
- 201-114-5
- EC Name:
- Triethyl phosphate
- Cas Number:
- 78-40-0
- Molecular formula:
- C6H15O4P
- IUPAC Name:
- triethyl phosphate
- Details on test material:
- purity: 98%
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3333, 10000 µg/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide, 9-aminoacridine, 4-nitro-o-phenylenediamine, mitomycon C, sodium azide, 9-aminoacridine, 4-nitro-o-phenylenediamine, 2-aminoanthracene
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Ames test was negative (-) at SRI
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
An Ames test was conducted using a preincubation protocol in the absence of exogenous metabolic activation, and in the presence of liver S-9.
Result: triethyl phosphate was negative in the test with and without metabolic activation.
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