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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
endocrine system modulation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Assessing in-vitro estrogenic effects of currently-used flame retardants
Author:
Krivoshiev BV, Dardennea F, Covacib A, Blusta R, Hussona SJ
Year:
2016
Bibliographic source:
Toxicology in Vitro 33, 153-162

Materials and methods

Principles of method if other than guideline:
Proliferation of the estrogen receptor α-positive human breast cancer cell line, MCF-7, has been routinely used as a measure of estrogenicity.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
content: 99.99%

Administration / exposure

Details on study design:
The MCF-7 flow cytometry cell proliferation assay has been described previously (Vanparys et al., 2006). Briefly, 50,000 cells were seeded into 6-well plates and allowed to attach. Following cell-cycle synchronisation in exposure medium for 48 h, compounds were added and cells treated for 24 h. Depending on endpoint measured (anti-estrogenicity or estrogenicity) cells were either co-treated with orwithout 17β-estradiol (E2) (100 pM). The proliferative potential of a FR compound was indicated by the number of cells in the DNA synthesis stage of the cell cycle, the S-phase. Results are presented as the mean value of three independent experiments.

Results and discussion

Details on results:
Triethyl phosphate was tested in the estrogen receptor α-positive human breast cancer cell line MCF-7 for the induction of cell proliferation, a screening assay for potential estrogenic effects. The concentrations tested were reported to exert no relevant cytotoxicity. It was shown in this study that triethyl phosphate induces no cell prolifereration in MCF-7 cells. No anti-estrogenic activity was seen for triethyl phosphate.

Applicant's summary and conclusion