Registration Dossier

Toxicological information

Genetic toxicity: in vivo

Currently viewing:

Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: sufficent documented for evaluation

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Cytogenetic effects induced by organophosphorus pesticides in mouse spermatocytes
Author:
Degraeve N, Chollet AC, Moutschen J
Year:
1984
Bibliographic source:
Toxicol Lett 21, 315-319
Reference Type:
publication
Title:
Mutagenic effects induced by organophosphorus compounds
Author:
Degraeve N, Chollet MC, Moutschen J
Year:
1986
Bibliographic source:
J Toxicol Clin Exp

Materials and methods

Principles of method if other than guideline:
Male mice (Q-strain) received a single ip injection of triethyl phosphate. The cytogenetic effects were investigated in bone marrow cells and spermatogonia.
GLP compliance:
not specified
Type of assay:
chromosome aberration assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Triethyp phosphate was suplied by Merck-Schuchardt

Test animals

Species:
mouse
Strain:
other: Q-Strain
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Duration of treatment / exposure:
12 to 36 hours or 10 to 15 days
Frequency of treatment:
single injection
Post exposure period:
no
Doses / concentrations
Remarks:
Doses / Concentrations:
300 mg/kg bw
Basis:
nominal conc.
No. of animals per sex per dose:
20 animals
Control animals:
yes

Examinations

Tissues and cell types examined:
bone marrow cells and spermatogonia

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Negative controls validity:
valid
Positive controls validity:
valid

Any other information on results incl. tables

The frequency of chromosome aberrations observed in bone marrow metaphases and spermatogonia of the animals treated with triethyl phophate did not increase significantly with respect to the control levels

Applicant's summary and conclusion

Executive summary:

Male mice (Q-strain) received a single ip injection of triethyl phosphate. The cytogenetic effects were investigated in bone marrow cells and spermatogonia.

The frequency of chromosome aberrations observed in bone marrow metaphases and spermatogonia of the animals treated with triethyl phophate did not increase significantly with respect to the control levels.