Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March - August 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to international guidelines and GLPs using the commercial product as test article.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2001
Reference Type:
publication
Title:
Unnamed
Year:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Test material was a composite of equal porportions of the commercial DecaBDE products of Albemarle Corp, Dead Sea Bromine Group, and Chemtura Corp.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 +-2 degrees C

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

Treatment

Concentration (mg/L)

Respiration Rate (mg O2/L/hr)

Percent

Inhibition

Control 1

NA

41.6

NA

Control 2

NA

41.6

NA

3,5-dichlorophenol

3

39.0

6.3

3,5-dichlorophenol

15

12.8

69.2

3,5-dichlorophenol

50

4.9

88.2

DecaBDE

15

40.0

3.9

DecaBDE

15

43.4

-4.3

DecaBDE

15

40.0

3.9

NA: Not applicable.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Difference in respiration between the 2 controls < 15% differece established for the test.
Conclusions:
DecaBDE did not inhibit the respiration of activiated sewage sludge.
Executive summary:

DecaBDE did not inhibit the respiration of activiated sewage sludge when tested at a concentration of 15 mg/L. The negative and positve controls performed as expected.