Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was reviewed as a part of the EU's RAR of DecaBDE, and accepted.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Responses of marine unicellular algae to brominated organic compounds in six growth media.
Author:
Walsh et al.
Year:
1987
Bibliographic source:
Ecotoxoxicology and Environmental Safety 14,215 –222.
Reference Type:
other: EU RAR
Title:
Unnamed
Year:
2002

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The effect of growth media on repsonse of algae to the test article was investigaged.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
other: S. costatum (72 hr), T. pseudonana (72 hr) , Chlorella sp (96 hr).

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
also 96 h
Post exposure observation period:
no

Test conditions

Hardness:
see below
Test temperature:
see below
pH:
see below
Dissolved oxygen:
see below
Salinity:
see below
Nominal and measured concentrations:
0, 1 mg/L
Details on test conditions:
see below
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
cell number
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
cell number
Details on results:
see below

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
DecaBDE was not toxic to 3 marine algae - S. costatum (72 hr), T. pseudonana (72 hr) , Chlorella sp (96 hr). - when tested at 1 mg/L.
Executive summary:

Walsh et al. (1987) studied the toxicity of DecaBDE to the marine unicellular algaeSkeletonema costatum,Thalassiosira pseudonanaandChlorellasp. The tests were carried out at a salinity of 30

for either 72 hours (S. costatumandT. pseudonana) or 96 hours (Chlorellasp.). The end‑point measured was the EC50for growth based on cell numbers. The exposure concentrations in the test solutions were verified by analysis. In the tests, the DecaBDE was added as a solution in acetone (final acetone concentration around 1 ml/l). Six different growth media were used in the test, one natural seawater and five synthetic seawater formulations. The natural seawater had a salinity of 32

and was diluted to give a final test salinity of 30

to be comparable with that of the synthetic media. The pHs of the various test media were in the range 7.6‑8.2. The EC50 for all three species was greater than the highest concentration tested (1 mg/l).