Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March - Dec 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Performed according to guidelines and GLPs using commercial product as test article.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2001
Reference Type:
publication
Title:
Unnamed
Year:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
Qualifier:
according to
Guideline:
other: OECD Proposed Guideline for Earthworm Reproduction Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Test material was a composite of equal porportions of the commercial DecaBDE products of Albemarle Corp, Dead Sea Bromine Group, and Great Lakes Chemical Corp

Sampling and analysis

Analytical monitoring:
yes

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
56 d
Post exposure observation period:
None.

Test conditions

Test temperature:
16.8 - 20.7 degrees C
pH:
soil pH Day 0 = 5.86 to 6.07
soil pH Day 56 = 5.35 to 5.86
Moisture:
Day O = 25.3 to 28.2%
Day 56 = 36.4 to 45.3%
Nominal and measured concentrations:
Nominal: 0 (Control), 312.6, 650.0, 1250, 2500 and 5000 mg DecaBDE/kg dry soil
Mean Measured: <100 (Control), 320, 668, 1240, 2480 and 4910 mg DecaBDE/kg dry soil
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 4 910 mg/kg soil dw
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: survival
Duration:
56 d
Dose descriptor:
EC50
Effect conc.:
> 4 910 mg/kg soil dw
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
4 910 mg/kg soil dw
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: survival and reproduction

Any other information on results incl. tables

At the end of the study, all earthworm tissue concentrations of DecaBDE, regardless of what test dose concentration was analyzed, were belwo the level of quantification (<0.75 ug/g tissue).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
DecaBDE incorporated into soil at nominal concentrations of up to 5000 mg/kg dry weight soil had no effect on the 56 d surival or reproduction of the earthworm, E. fetida. The NOEC for survival and reproduction was 5000 mg/kg dw (nominal) or 4910 mg/kg dw (measured). DecaBDE was not detected in earthworm tissue at the end of the study (LOQ=0.75 ug/g tissue).
Executive summary:

DecaBDE incorporated into soil at nominal concentrations of up to 5000 mg/kg dry weight soil had no effect on the 56 d surival or reproduction of the earthworm, E. fetida. The NOEC for survival and reproduction was 5000 mg/kg dw (nominal) or 4910 mg/kg dw (measured). DecaBDE was not detected in earthworm tissue at the end of the study (LOQ=0.75 ug/g tissue).