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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, sufficiently documented and acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report Date:
1977

Materials and methods

Principles of method if other than guideline:
According to: Smyth et al.: Am. Ind. Hyg. Ass. J. 23: 95-107 (1962). Volatile part of compound is directed via airstream (200L/h) into inhalation chamber with a single rat. 12 rats exposed. Exposure times: 3 and 7 hours. The time is determined at which all animals survive. Monitoring time after exposition is 14 days
GLP compliance:
no
Test type:
other: Inhalation risk test
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): nitrobenzene
- Analytical purity: no data

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Analytical verification of test atmosphere concentrations:
not specified
Remarks on duration:
3 and 7 hours
Concentrations:
mean concentration: 2.27 mg/L (7h) and 2.25 mg/L (3h)
No. of animals per sex per dose:
single rat per exposition; 12 rats per exposure time
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC0
Effect level:
2.25 mg/L air
Based on:
other: mean concentration of test material
Exp. duration:
3 h
Sex:
male/female
Dose descriptor:
other: 3/12 rats died after 7h exposition
Effect level:
2.27 mg/L air
Based on:
other: mean concentration of test material
Exp. duration:
7 h

Any other information on results incl. tables

Mortality: 0/12 deaths after 3h exposition. 3/12 rats died after 7h exposition at 20°C. After 7 h exposition, animals were gasping for breath, had severe irritation of mucouse membrane and

cyanosis as well as disturbed equilibrium.

Animals that died during the study showed acute dilation and congestion of the heart, organs had discolorations and the lung was inflated and filled with blood.

Applicant's summary and conclusion