Registration Dossier
Registration Dossier
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EC number: 202-716-0 | CAS number: 98-95-3
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically acceptable. Guideline study, well documented and with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Reference Type:
- secondary source
- Title:
- European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
- Author:
- European Chemicals Bureau
- Year:
- 2 007
- Bibliographic source:
- 3rd Priority List, Volume: 77
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: "Proposed Preliminary Testing Method: prolonged toxicity test on Daphnia magna", according to the Federal Environmental Agency .
- GLP compliance:
- no
Test material
- Reference substance name:
- Nitrobenzene
- EC Number:
- 202-716-0
- EC Name:
- Nitrobenzene
- Cas Number:
- 98-95-3
- Molecular formula:
- C6H5NO2
- IUPAC Name:
- nitrobenzene
- Details on test material:
- - Analytical purity: 99 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: GC or HPLC, combined with FID ,ECD , FPD or MS
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution series of the stock solution
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: IRCHA
- Source: Federal Envrionmental Agency
- Feeding during test
- Food type: Algae and TetraMin
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- pH:
- 7.45 - 7.80
- Dissolved oxygen:
- Saturation of oxygen: about 77 - 90 %
- Nominal and measured concentrations:
- Nominal test concentrations: 1.56, 3.13, 6.25, 12.5, 25, 50, 100 and 200 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels filled with 250 ml test substance
- Type (delete if not applicable): open
- Renewal rate of test solution (frequency/flow rate): every 3 days
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater (according to DIN)
OTHER TEST CONDITIONS
- Photoperiod: 9:15 (light:dark)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Analytical determined value
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
Any other information on results incl. tables
Recovery rates: 12.5 mg/L (nominal): 21 -29 % recovery 25.0 mg/L (nominal): 14 -22 % recovery 50.0 mg/L (nominal): 10 -26 % recovery
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
