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Toxicological information

Neurotoxicity

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Administrative data

Endpoint:
neurotoxicity: sub-chronic inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, however, limited documentation

Data source

Reference
Reference Type:
publication
Title:
Selective degeneration in the rat olfactory bulb following exposure to different odours
Author:
Pinching AJ, Doving KB
Year:
1974
Bibliographic source:
Brain Research 82: 195-204

Materials and methods

Principles of method if other than guideline:
Animals were exposed to constant nitrobenzene vapour. The animals were sacrificed at about 1, 2 and 3 months of age. As to control, animals exposed to filtered fresh air only.

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Nitrobenzene
- Molecular formula (if other than submission substance): C6H5NO2
- Molecular weight (if other than submission substance): 123

Test animals

Species:
rat
Strain:
Wistar
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: 2 weks
- Weight at study initiation: 28 - 39 g
- Fasting period before study: no dat

Administration / exposure

Route of administration:
inhalation: vapour
Vehicle:
other: room air filtered through filters of charcoal and molecular sieves
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
5 or 10 weeks
Sacrifice after 5 and 10 weeks, respectively
Frequency of treatment:
continuously constant
Doses / concentrations
Remarks:
Doses / Concentrations:
5.4E-111M
Basis:
other: Difference of the weight between before and after the experiment.
No. of animals per sex per dose:
4 totally, no data about the sexuality of animals tested
Control animals:
yes, concurrent vehicle

Results and discussion

Any other information on results incl. tables

Microscopic examination of the olfactory bulb revealed a degeneration of the mitral cell layer, representing the principal relay neurones, with the most densely degeneration in the ventral region.

Applicant's summary and conclusion