Registration Dossier

Administrative data

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
"The effect values found are usually very low compared to effect values found by other authors. No explanation for these large discrepancies could be found. It was not possible to reproduce the effect values found in this study. Hence, it was decided not to use these data for a derivation of a PNECaqua if other valid fish early life stage tests are available." From EU-RAR, Nitrobenzene, 2007

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1982
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Aquatic Toxicitiy of Organic Compounds to Embryo-Larval Stages of Fish and Amphibians
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
4 days post-hatching

Test conditions

Hardness:
112.1 ± 1.2 mg/L CaCO3
Test temperature:
14.2 ± 0.1°C
pH:
7.9 ± 0.02
Dissolved oxygen:
9.8 ± 0.1 mg/L

Results and discussion

Effect concentrations
Duration:
23 d
Dose descriptor:
LC50
Effect conc.:
0.002 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
no