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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, acceptable for assessment.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Comparative toxicity of ten organic chemicals to four earthworm species
Author:
Neuhauser et al.
Year:
1986
Bibliographic source:
Comp. Pharmacol. Toxicol. 83: 197-200
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Development of a standardized toxicity laboratory method for assessing the toxicity of chemical substances to earthworms. Report EUR 8714EN. Com- Acknowledgement-This work was supported by a grant mission of theCommission of the European Community.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: >98%

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone or chloroform (if used!)

Test organisms

Test organisms (species):
other: Eisenia fetida, Allolobophora tuberculata, Eudrilus eugeniae, Perionyx excavatus
Animal group:
annelids
Details on test organisms:
- Housing conditions: at room temperature (20 -22 °C) on a consistent organic food source (horse manure)

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d

Test conditions

Test temperature:
20 °C
pH:
6.0 ± 0.5
Moisture:
35% dry wt
Details on test conditions:
TEST SYSTEM
- Test container (material, size): covered glass dishes, 6.5 cm high and 12.5 cm in diameter
- Amount of soil or substrate: 400g dry wt
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE
Artificial soil: consisted by weight of 10% finely ground sphagnum peat, 20% kaolinite clay, 69% fine sand and 1% pulverized calcium carbonate.
Water content: 35% of the dry wt of the four components

EFFECT PARAMETERS MEASURED: mortality after 14 days

TEST CONCENTRATIONS
- Range finding study: yes
Nominal and measured concentrations:
- Five chemical concentrations

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
319 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Eisenia fetida
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
226 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Eudrilus eugeniae
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
343 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Perionyx excavatus
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
362 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Species: Allolobophora tuberculata
Reported statistics and error estimates:
LC50 values were calculated using the method of Litchfield and Wilcoxon, 1949

Applicant's summary and conclusion

Validity criteria fulfilled:
yes