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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1992
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Sugishima fish farm, Japan
- Length at study initiation (lenght definition, mean, range and SD): ca. 10 cm
- Weight at study initiation (mean and range, SD): ca. 30 g
- Method of breeding: Pelleted feed for carp

ACCLIMATION
- Acclimation period: 28 days
- Acclimation conditions (same as test or not): 25 °C +/- 1 °C

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
42 d

Test conditions

Test temperature:
25 °C
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: glass tanks of 100 liters in volume
- Type of flow-through (e.g. peristaltic or proportional diluter): Aquatron
- Renewal rate of test solution (frequency/flow rate): 200-800 ml/min
- No. of organisms per vessel: 15 - 20
- Source/preparation of dilution water: Dilution water used for the test originated from underground water pumped up from the ground of Kurume Research Laboratories.
- Intervals of water quality measurement: twice a week

TEST SYSTEM
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: glass tanks of 100 liters in volume
- Type of flow-through (e.g. peristaltic or proportional diluter): Aquatron
- Renewal rate of test solution (frequency/flow rate): 200-800 ml/min
- No. of organisms per vessel: 15 - 20

Test medium/ water parameters
- Source/preparation of dilution water: Dilution water used for the test originated from underground water pumped up from the ground of Kurume Research Laboratories.
- Intervals of water quality measurement: twice a week
Nominal and measured concentrations:
0.125 mg/l
0.0125 mg/l

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
ca. 3.1 - ca. 4.8
Basis:
other: Concentration of test substance in fish, no further data available.
Remarks on result:
other: Conc.in environment / dose:0.125 mg/L
Type:
BCF
Value:
ca. 1.6 - ca. 7.7
Basis:
other: Concentration of test substance in fish, no further data available.
Remarks on result:
other: Conc.in environment / dose:0.0125 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes