Nitrobenzene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with slight modifications according to Van Leeuwen et al. (1985). Limited documentation.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1982

Report date:

1982

Reference 2

Reference Type:

secondary source

Title:

European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3

Author:

European Chemicals Bureau

Year:

2007

Bibliographic source:

3rd Priority List, Volume: 77

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

no

Remarks:

test prior to 1 June 2008

Test material

Test material information

Constituent 1

Reference substance name:

Nitrobenzene

EC Number:

202-716-0

EC Name:

Nitrobenzene

Cas Number:

98-95-3

Molecular formula:

C6H5NO2

IUPAC Name:

nitrobenzene

Details on test material:

- Analytical purity: No data about the degree of purity of the test substance were provided.
DMSO (purity 99%) was used as vehicle for the preparation of the stock solution.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Chlorella pyrenoidosa

Details on test organisms:

Growing Chlorella pyrenoidosa

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

18 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes