Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study, which meets basic scientific principles. Method followed OECD guideline.
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Toxicity, biodegradability, and accumulation of a number of CI/N-containing compounds for classification and establishing Water Quality Criteria
Author:
Canton JH et al.
Year:
1985
Bibliographic source:
Reg Toxicol Pharm 5: 123-131
Report Date:
1985
Reference Type:
publication
Title:
Toxicity and Accumulation Studies of Cadmium with Freshwater Organisms of Different Trophic Levels
Author:
Canton JH and Slooff W
Year:
1982
Bibliographic source:
Ecotox. Environ. Safety 6:113-128
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: according to OECD proposal, 1979
Principles of method if other than guideline:
Additional details on test procedure not given in Canton et al. (1985) are given in Canton and Slooff (1982) referenced above. Procedures were conductedin accordance with proposed OECD guideline of 1979; however, additional details are not available on exposure concentrations, geometric, spacing thereof, or replication of exposures.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Substance obtained from Fluka, with purity determined to be > 99.9% by gas chromatography.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
In a preliminary test, loss of the test substance from test medium (open vessel) was determined after 1, 2, 4, and 8 days. Recovery of the test substance was 96% after 48 hrs., and 92% after 96 hrs. Therefore, static exposure conditions were determined to be sufficient for the 48 hr. exposure to D. magna, and additional analytical verification was not deemed necessary.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): <=24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
not applicable

Test conditions

Hardness:
2 mM as CaCO3 (Dutch Standard Water, DSW), containing:
100 mg/L NaHCO3
200 mg/L CaCL2*2H2O
20 mg/L KHCO3
180 mg/L MgSO4*7H2O
as described in Canton and Slooff, 1982
Test temperature:
19 °C
pH:
Not reported
Dissolved oxygen:
Not reported. The static exposures were open to atmosphere without aeration.
Salinity:
Freshwater (formulated as described above)
Nominal and measured concentrations:
Exposure concentrations, and geometric spacing thereof, are not given in the report. However, reported effect concentrations are corrected for recovery (96%) demonstrated for test substance determined in preliminary experiment.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
35 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
62 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Not available

Any other information on results incl. tables

Reported effect concentrations (EC50 and LC50) corrected for recovery of test substance (96%) determined after 48 hrs. in preliminary recovery experiment.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Guideline test
Conclusions:
The 48 hr. EC50 with Daphnia magna is 35 mg/L
The 48 hr LC50 with Daphnica magna is 62 mg/L