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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
Unnamed
Year:
1990
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Nitrobenzol
- Analytical purity: no data available
- Impurities (identity and concentrations): no data available

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:
- Sampling method: GC

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution for every concentration
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): max. 158 mg/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc):

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish
- Source: Doller Zierfischzüchterei, Finnentrop-Serkenrode, Germany
- Age at study initiation (mean and range, SD): > 12 d
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 1 cm

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test?): yes
- Type and amount of food: fed daily with TetraMin

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
23° +/- 1°C
pH:
8.15 +/- 0.2
Nominal and measured concentrations:
Conc. nom.: 240.7, 120.4, 60.2, 30.1 and 15.04 mg/L
Conc. analyt.: 98, 89.9, 40.2, 26.6 and 9.9 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 11 l
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): dosing of test substance by use of a perfusor
- Renewal rate of test solution (frequency/flow rate): 6 times per day
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
92 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
44 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
98 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion