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EC number: 701-197-2
CAS number: -
Activated sewage sludge, OECD 209: EC50(3h) > 1000 mg/L, NOEC(3h): 1000 mg/L
Activated sewage sludge, obtained from a
predominantly domestic sewage, was exposed to the test substance to
investigate the toxic effects towards aquatic microorganisms (Wadsley,
2012). The suspended solid concentration was determined as 3 g/L prior
to the start of the experiment. After stock solution preparation, the
25, 250 and 2500 mg/L stock solution were used to prepare the test
concentrations of 10, 100 and 1000 mg/L. The 1000 mg/L test
concentration was prepared in triplicate. Synthetic sewage acted as a
respiratory substrate. 3,5-dichlorophenol was used as reference item and
resulted in an EC50(3h) of 14 mg/L and confirmed thereby the suitability
of the inoculum. The control group (three replicates) were maintained
under identical conditions but not exposed to the test item. The test
was performed in a temperature controlled room at 20 +/- 2 °C under
normal laboratory lighting with continuous illumination. The oxygen
concentrations in all vessels were measured after 30 minutes contact
time. The test item vessels thereby oxygen concentrations between 45 to
70 % of saturation. This deviation was considered to have had no adverse
effect on the study given that all oxygen consumption values were
measured/calculated over the linear portion of the traces. No
statistically significant toxic effects were shown at all of the test
concentration employed in the range-finding study. It was therefore
considered justifiable not to perform a definitive test. The EC50(3h) is
greater than 1000 mg/L with the corresponding NOEC of 1000 mg/L. It was
considered unnecessary and unrealistic to test at concentrations in
excess of 1000 mg/L.
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