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EC number: 701-197-2
CAS number: -
Algal growth inhibition test_Pseudokirchneriella subcapitata_OECD 201/EU Method C.3: ErC10(72h), ErC20(72h) and ErC50(72h) > 120 mg/L / EbC10(72h): 7.7 mg/L, EbC20(72h): 120 mg/L (extrapolated) and EbC50(72h) > 120 mg/L (extrapolated). NOEC(72h): 21 mg/L. NOEC(72h): 69 mg/L Calculation with ECOSAR v0.99g and v1.00 (EPIWIN software by US-EPA): Main component with chlorine: EC50(96h): 14324 mg/L; ChV(4d): 11676.9 mg/L Main component without chlorine: EC50(96h): 33823 mg/L; ChV(4d): 12795.8 mg/L
The effects of 1,2,3-Propanetriol, glycidyl
ethers on the growth of the freshwater green algal species Pseudokirchneriella
subcapitata was investigated according to OECD Guideline 201 / EU
Method C.3 (Liedtke, 2013). The study was conducted under certificated
GLP compliance. Exponentially growing cultures of the test organisms
were exposed to the different concentrations of the test substance over
a period of 72 hours and the inhibition of algal growth in relation to
control cultures was assessed over several generations. The selection of
the test concentrations was based on the results of a range-finding test
and on results of a pre-experiment to determine the solubility of the
test substance. The following nominal concentrations were chosen: 0.32,
1.0, 3.2, 10, 32 and 100 mg/L (three replicates per concentration).
Additionally, a control was tested in parallel (six replicates). The
initial cell density was 5000 cells/mL (nominal). A static test design
was applied. A small volume of the algal suspension was withdrawn daily
from each test flask for the measurement of the biomass, and was not
replaced. The concentrations of the test substance were determined in
one of the duplicate test medium samples from the nominal test
concentrations of 32 and 100 mg/L. The samples from the nominal test
concentrations of 0.32 to 10 mg/L were not analyzed, since these
concentrations were below the NOEC determined in this test. From the
control samples, one of the duplicate samples was analyzed per sampling
time. The measured concentrations in the test media of the nominal test
concentrations of 32 and 100 mg/L were 77 and 79 % of the nominal values
at the start of the test, respectively. During the test period of 72
hours, a decrease of test item concentration in the test media occurred.
At the end of the test, 58 and 61 % of the nominal values were found,
respectively. The microscopic examination of the algal cells at the end
of the test showed no difference between the algae growing at the
nominal test concentration of 100 mg/L and the algal cells in the
control. The shape and size of the algal cells were obviously not
affected by the test item up to at least this concentration. No
remarkable observations were made concerning the appearance of the test
media. All test media were clear solutions throughout the test period.
The biological results based on growth rate can be summarized as
follows: ErC10(72h), ErC20(72h) as well as ErC50(72h) were found to be >
120 mg/L. Based on yield, the results were reported as: EbC10(72h): 7.7
mg/L, EbC20(72h): 120 mg/L (extrapolated) and EbC50(72h) > 120 mg/L
(extrapolated). The NOEC was found as 21 mg/L and the NOEC as 69 mg/L
after 72 hours exposure duration, respectively. Defined values for EC10
(growth rate), EC20 and EC50 (growth rate and yield) could not be
calculated due to the low toxicity of the test item for the algae.
Confidence intervals could not be calculated.
information, the acute and chronic toxicity to green algae (species not
further specified) of the test substance was predicted by the computer
program ECOSAR v0.99g and v1.00 (EPIWIN software) by US-EPA (Chemservice
S.A., 2012; Chemservice S.A., 2015c). An extensive set of structure –
activity relationships (SARs) is utilized by the computer program, to
predict the acute and chronic toxicity for three trophic levels for
aquatic species, based on the ECOSAR-classes of “Diepoxides” (v0.99g)
and “Epoxides, Poly” (v1.00). For the main component with chlorine, an EC50
of 14324 mg/L is predicted after a test substance exposure of 96 h to
green algae. Concerning the main component without chlorine, the EC50 is
predicted as 33823 mg/L. Regarding long-term toxicity, the so-called
Chronic Value (ChV) is predicted, which is defined as the geometric mean
of the Lowest Observed Effect Concentration (LOEC) and the No Observed
Effect Concentration (NOEC). It can just classify a substance according
to a low, moderate or high toxicity concern. For the test substance
concerning long-term toxicity to green algae a ChV(4d) of 11676.9 mg/L
is predicted for the main component with chlorine and a value of 12795.8
mg/L for the main component without chlorine. Both values indicate that
there is no toxicity concern.
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